G6PD Monoclonal antibody

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  WB analysis of HeLa using 66373-1-Ig

  WB result of G6PD antibody (66373-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G6PD transfected HeLa cells.

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  WB analysis of HepG2 using 66373-1-Ig

  Several cell lines were subjected to SDS PAGE followed by western blot with 66373-1-Ig (G6PD Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 66373-1-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66373-1-Ig (G6PD Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66373-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66373-1-Ig (G6PD Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66373-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66373-1-Ig (G6PD Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66373-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using G6PD antibody (66373-1-Ig, Clone: 2A7B12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of HeLa using 66373-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66373-1-Ig (G6PD antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• Featured Product
• KD/KO Validated

G6PD Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66373-1-Ig

Clone No.2A7B12

Publications(14)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

2A7B12, EC:1.1.1.49, G6P, G6PD1, Glucose-6-phosphate 1-dehydrogenase

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, rat spleen tissue, L02 cells, HEK-293 cells, NIH/3T3 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 5 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

66373-1-Ig targets G6PD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21862

  Product name: Recombinant human G6PD protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-515 aa of BC000337

  Sequence: MAEQVALSRTQVCGILREELFQGDAFHQSDTHIFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKATPEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMNALHLGSQANRLFYLALPPTVYEAVTKNIHESCMSQIGWNRIIVEKPFGRDLQSSDRLSNHISSLFREDQIYRIDHYLGKEMVQNLMVLRFANRIFGPIWNRDNIACVILTFKEPFGTEGRGGYFDEFGIIRDVMQNHLLQMLCLVAMEKPASTNSDDVRDEKVKVLKCISEVQANNVVLGQYVGNPDGEGEATKGYLDDPTVPRGSTTATFAAVVLYVENERWDGVPFILRCGKALNERKAEVRLQFHDVAGDIFHQQCKRNELVIRVQPNEAVYTKMMTKKPGMFFNPEESELDLTYGNRYKNVKLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTPLLHQIELEKPKPIPYIYGSRGPTEADELMKRVGFQYEGTYKWVNPHKL

• Full Name: glucose-6-phosphate dehydrogenase
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC000337
• Gene Symbol: G6PD
• Gene ID (NCBI): 2539
• RRID: AB_2877122
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11413
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	IP,IHC,IF	Cell Metab; Upregulation of Antioxidant Capacity and Nucleotide Precursor Availability Suffices for Oncogenic Transformation.; Authors - Yang Zhang
rat,mouse	WB	Redox Biol; The E3 ubiquitin ligase TRIM31 is involved in cerebral ischemic injury by promoting degradation of TIGAR.; Authors - Shenglan Zeng
mouse,human	WB,IHC	Cell Rep; Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation; Authors - Zehe Ge
mouse	WB	Clin Transl Med; SIRT5 deficiency enhances the proliferative and therapeutic capacities of adipose-derived mesenchymal stem cells via metabolic switching.; Authors - Tiantong Ou
mouse	WB	Int J Mol Sci; G6PD Deficiency Is Crucial for Insulin Signaling Activation in Skeletal Muscle.; Authors - Aiwen Jiang ; KD Validated
human	WB	J Pharmacol Exp Ther; Mechanistic Analysis of an Extracellular Signal-Regulated Kinase 2-Interacting Compound that Inhibits Mutant BRAF-Expressing Melanoma Cells by Inducing Oxidative Stress.; Authors - Ramon Martinez