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  • KD/KO Validated

G6PD Monoclonal antibody

G6PD Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2A7B12

Cat no : 66373-1-Ig

Synonyms

G6PD, G6PD1



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, rat spleen tissue, L02 cells, HEK-293 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:200
Sample-dependent, check data in validation data gallery

Product Information

66373-1-Ig targets G6PD in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen G6PD fusion protein Ag21862
Full Name glucose-6-phosphate dehydrogenase
Calculated molecular weight 59 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC000337
Gene symbol G6PD
Gene ID (NCBI) 2539
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for G6PD antibody 66373-1-IgDownload protocol
IHC protocol for G6PD antibody 66373-1-IgDownload protocol
IF protocol for G6PD antibody 66373-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC

FASEB J

High glucose-induced ubiquitination of G6PD leads to the injury of podocytes.

Authors - Meng Wang
  • KD Validated
human,mouseWB

Front Physiol

Progesterone Regulates Glucose Metabolism Through Glucose Transporter 1 to Promote Endometrial Receptivity.

Authors - Hongshuo Zhang
mouseWB

Clin Transl Med

SIRT5 deficiency enhances the proliferative and therapeutic capacities of adipose-derived mesenchymal stem cells via metabolic switching.

Authors - Tiantong Ou
mouse,humanIHC,IF,IP

Cell Metab

Upregulation of Antioxidant Capacity and Nucleotide Precursor Availability Suffices for Oncogenic Transformation.

Authors - Yang Zhang