G6PD Monoclonal antibody, PBS Only

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  WB analysis of HeLa using 66373-1-Ig (same clone as 66373-1-PBS)

  WB result of G6PD antibody (66373-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G6PD transfected HeLa cells. This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66373-1-Ig (same clone as 66373-1-PBS)

  Several cell lines were subjected to SDS PAGE followed by western blot with 66373-1-Ig (G6PD Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

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  WB analysis of rat spleen using 66373-1-Ig (same clone as 66373-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66373-1-Ig (G6PD Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66373-1-Ig (same clone as 66373-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66373-1-Ig (G6PD Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66373-1-Ig (same clone as 66373-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66373-1-Ig (G6PD Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66373-1-Ig (same clone as 66373-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using G6PD antibody (66373-1-Ig, Clone: 2A7B12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66373-1-Ig (same clone as 66373-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66373-1-Ig (G6PD antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

G6PD Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66373-1-PBS

Clone No.2A7B12

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

2A7B12, EC:1.1.1.49, G6P, G6PD1, Glucose-6-phosphate 1-dehydrogenase

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Product Information

66373-1-PBS targets G6PD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21862

  Product name: Recombinant human G6PD protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-515 aa of BC000337

  Sequence: MAEQVALSRTQVCGILREELFQGDAFHQSDTHIFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKATPEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMNALHLGSQANRLFYLALPPTVYEAVTKNIHESCMSQIGWNRIIVEKPFGRDLQSSDRLSNHISSLFREDQIYRIDHYLGKEMVQNLMVLRFANRIFGPIWNRDNIACVILTFKEPFGTEGRGGYFDEFGIIRDVMQNHLLQMLCLVAMEKPASTNSDDVRDEKVKVLKCISEVQANNVVLGQYVGNPDGEGEATKGYLDDPTVPRGSTTATFAAVVLYVENERWDGVPFILRCGKALNERKAEVRLQFHDVAGDIFHQQCKRNELVIRVQPNEAVYTKMMTKKPGMFFNPEESELDLTYGNRYKNVKLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTPLLHQIELEKPKPIPYIYGSRGPTEADELMKRVGFQYEGTYKWVNPHKL

• Full Name: glucose-6-phosphate dehydrogenase
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC000337
• Gene Symbol: G6PD
• Gene ID (NCBI): 2539
• RRID: AB_2877122
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11413
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.