Validation Data Gallery
|Positive WB detected in||COLO 320 cells, rat colon tissue, NIH/3T3 cells, human heart tissue, HeLa cells, MCF-7 cells|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human thyroid cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
14979-1-AP targets Galectin-3 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Galectin-3 fusion protein Ag6891|
|Full Name||lectin, galactoside-binding, soluble, 3|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||31 kDa|
|GenBank accession number||BC001120|
|Gene ID (NCBI)||3958|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a 31-kDa member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).
|Product Specific Protocols|
|WB protocol for Galectin-3 antibody 14979-1-AP||Download protocol|
|IHC protocol for Galectin-3 antibody 14979-1-AP||Download protocol|
|IF protocol for Galectin-3 antibody 14979-1-AP||Download protocol|
|IP protocol for Galectin-3 antibody 14979-1-AP||Download protocol|
|FC protocol for Galectin-3 antibody 14979-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Agric Food Chem
Synephrine Hydrochloride Suppresses Esophageal Cancer Tumor Growth and Metastatic Potential through Inhibition of Galectin-3-AKT/ERK Signaling.
Front Cell Dev Biol
Activation of Galectin-3 (LGALS3) Transcription by Injurious Stimuli in the Liver Is Commonly Mediated by BRG1.
Galectin-3 Induces Atrial Fibrosis by Activating the TGF-β1/Smad Pathway in Patients with Atrial Fibrillation.
Assessment of mitochondrial function in metabolic dysfunction-associated fatty liver disease using obese mouse models.
J Am Soc Hypertens
Galectin-3 mediates the pulmonary arterial hypertension-induced right ventricular remodeling through interacting with NADPH oxidase 4.
Initial human T-cell leukemia virus type 1 infection of the salivary gland epithelial cells requires a biofilm-like structure.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tom (Verified Customer) (07-07-2021)
HEK293 whole cell lysates on SDS-PAGE. Antibody incubated with membrane overnight in fridge. HRP secondary antibody used for detection.