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  • KD/KO Validated

Galectin-3 Polyclonal antibody

Galectin-3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14979-1-AP

Synonyms

35 kDa lectin, CBP 35, CBP35, Gal 3, GAL3, Galactose specific lectin 3, Galactoside binding protein, GALBP, Galectin 3, Galectin-3, GALIG, IgE binding protein, L 31, Laminin binding protein, Lectin L 29, LGALS2, LGALS3, Mac 2 antigen, MAC2



Tested Applications

Positive WB detected inCOLO 320 cells, rat colon tissue, NIH/3T3 cells, human heart tissue, HeLa cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman thyroid cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14979-1-AP targets Galectin-3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Galectin-3 fusion protein Ag6891
Full Name lectin, galactoside-binding, soluble, 3
Calculated molecular weight 26 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC001120
Gene symbol LGALS3
Gene ID (NCBI) 3958
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a 31-kDa member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).

Protocols

Product Specific Protocols
WB protocol for Galectin-3 antibody 14979-1-APDownload protocol
IHC protocol for Galectin-3 antibody 14979-1-APDownload protocol
IF protocol for Galectin-3 antibody 14979-1-APDownload protocol
IP protocol for Galectin-3 antibody 14979-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nature

A phosphoinositide signalling pathway mediates rapid lysosomal repair

Authors - Jay Xiaojun Tan
mouseWB

Nat Immunol

Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions.

Authors - Rui Martins
mouseIF

Br J Pharmacol

Substitution of the SERCA2 Cys(674) reactive thiol accelerates atherosclerosis by inducing endoplasmic reticulum stress and inflammation

Authors - Hang Su
mouseWB

Int J Biol Sci

Hgs Deficiency Caused Restrictive Cardiomyopathy via Disrupting Proteostasis.

Authors - Zhenhua Li
humanWB,IHC

Front Oncol

Prognostic Value and Biological Function of Galectins in Malignant Glioma.

Authors - Hongtao Zhu
humanWB,IF

Aging Dis

Galectin-3 Mediates Endothelial-to-Mesenchymal Transition in Pulmonary Arterial Hypertension.

Authors - Tangzhiming Li
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tom (Verified Customer) (07-07-2021)

HEK293 whole cell lysates on SDS-PAGE. Antibody incubated with membrane overnight in fridge. HRP secondary antibody used for detection.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293
Galectin-3 Antibody Western Blot validation (1:1000 dilution) in HEK293 (Cat no:14979-1-AP)