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Galectin-3 Polyclonal antibody
Galectin-3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 14979-1-AP
Validation Data Gallery
|Positive WB detected in||COLO 320 cells, rat colon tissue, NIH/3T3 cells, human heart tissue, HeLa cells, MCF-7 cells|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human thyroid cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
14979-1-AP targets Galectin-3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Galectin-3 fusion protein Ag6891|
|Full Name||lectin, galactoside-binding, soluble, 3|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||31 kDa|
|GenBank accession number||BC001120|
|Gene ID (NCBI)||3958|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).
|Product Specific Protocols|
|WB protocol for Galectin-3 antibody 14979-1-AP||Download protocol|
|IHC protocol for Galectin-3 antibody 14979-1-AP||Download protocol|
|IF protocol for Galectin-3 antibody 14979-1-AP||Download protocol|
|IP protocol for Galectin-3 antibody 14979-1-AP||Download protocol|
|FC protocol for Galectin-3 antibody 14979-1-AP||Download protocol|
|Click here to view our Standard Protocols|
A phosphoinositide signalling pathway mediates rapid lysosomal repair
Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions.
Br J Pharmacol
Substitution of the SERCA2 Cys(674) reactive thiol accelerates atherosclerosis by inducing endoplasmic reticulum stress and inflammation
Front Cell Dev Biol
Activation of Galectin-3 (LGALS3) Transcription by Injurious Stimuli in the Liver Is Commonly Mediated by BRG1.
J Agric Food Chem
Synephrine Hydrochloride Suppresses Esophageal Cancer Tumor Growth and Metastatic Potential through Inhibition of Galectin-3-AKT/ERK Signaling.
Int J Biol Sci
Hgs Deficiency Caused Restrictive Cardiomyopathy via Disrupting Proteostasis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tom (Verified Customer) (07-07-2021)
HEK293 whole cell lysates on SDS-PAGE. Antibody incubated with membrane overnight in fridge. HRP secondary antibody used for detection.