Galectin-3 Polyclonal antibody

• Featured Product
• KD/KO Validated

Galectin-3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

25

Reviews (1)

Cat no : 14979-1-AP

Synonyms

35 kDa lectin, CBP 35, CBP35, Gal 3, GAL3, Galactose specific lectin 3, Galactoside binding protein, GALBP, Galectin 3, Galectin-3, GALIG, IgE binding protein, L 31, Laminin binding protein, Lectin L 29, LGALS2, LGALS3, Mac 2 antigen, MAC2

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Validation Data Gallery

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  WB analysis using 14979-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa cells using 14979-1-AP

  WB result of Galectin 3 antibody (14979-1-AP, 1:1000) with si-Control and si-Galectin 3 transfected HeLa cells.

• 

  WB analysis of HeLa using 14979-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14979-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14979-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 14979-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 14979-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 14979-1-AP

  IP Result of anti-Galectin-3 (IP:14979-1-AP, 3ug; Detection:14979-1-AP 1:500) with MCF-7 cells lysate 1600ug.

• 

  IHC staining of human thyroid cancer using 14979-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14979-1-AP (Galectin-3 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human thyroid cancer using 14979-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14979-1-AP (Galectin-3 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 14979-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14979-1-AP (Galectin-3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 14979-1-AP

  Immunofluorescent analysis of MCF-7 cells using 14979-1-AP (Galectin-3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 14979-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Galectin-3 antibody (14979-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: COLO 320 cells, rat colon tissue, NIH/3T3 cells, human heart tissue, HeLa cells, MCF-7 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human thyroid cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 9 publications below
• IF: See 9 publications below
• IP: See 2 publications below

Product Information

14979-1-AP targets Galectin-3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Galectin-3 fusion protein Ag6891
• Full Name: lectin, galactoside-binding, soluble, 3
• Calculated molecular weight: 26 kDa
• Observed molecular weight: 31 kDa
• GenBank accession number: BC001120
• Gene symbol: LGALS3
• Gene ID (NCBI): 3958
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).

Publications

Species	Application	Title
human	IF	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan
mouse	WB	Nat Immunol; Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions.; Authors - Rui Martins
mouse	IF	Br J Pharmacol; Substitution of the SERCA2 Cys(674) reactive thiol accelerates atherosclerosis by inducing endoplasmic reticulum stress and inflammation; Authors - Hang Su
human,mouse	WB	Front Cell Dev Biol; Activation of Galectin-3 (LGALS3) Transcription by Injurious Stimuli in the Liver Is Commonly Mediated by BRG1.; Authors - Zilong Li
human	WB	J Agric Food Chem; Synephrine Hydrochloride Suppresses Esophageal Cancer Tumor Growth and Metastatic Potential through Inhibition of Galectin-3-AKT/ERK Signaling.; Authors - Wen Wen Xu
mouse	WB	Int J Biol Sci; Hgs Deficiency Caused Restrictive Cardiomyopathy via Disrupting Proteostasis.; Authors - Zhenhua Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (07-07-2021)

HEK293 whole cell lysates on SDS-PAGE. Antibody incubated with membrane overnight in fridge. HRP secondary antibody used for detection.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293

Galectin-3 Antibody Western Blot validation (1:1000 dilution) in HEK293 (Cat no:14979-1-AP)

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