GAPDH Polyclonal antibody

GAPDH Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, arabidopsis , corn , cabbage, rice and More (9)

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10494-1-AP

Synonyms

G3PD, GAPD, GAPDH, OK/SW cl.12



Tested Applications

Positive WB detected inhuman placenta tissue, HepG2 cells, HEK-293 cells, HeLa cells, Raji cells, K-562 cells, mouse heart tissue, PC-13 cells, arabidopsis whole plant tissue, corn whole plant tissue, mouse brain tissue, mouse skin tissue, Jurkat cells, NIH/3T3 cells, C6 cells, rat brain tissue, RAW 264.7 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:40000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

10494-1-AP targets GAPDH in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, arabidopsis , corn , cabbage, rice samples.

Tested Reactivity human, mouse, rat, pig, arabidopsis , corn , cabbage, rice
Cited Reactivityhuman, Arabidopsis, Bovine, Caenorhabditis elegans, canine, chicken, EPC cells, fish, goat, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAPDH fusion protein Ag0766
Full Name glyceraldehyde-3-phosphate dehydrogenase
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC004109
Gene symbol GAPDH
Gene ID (NCBI) 2597
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Protocols

Product Specific Protocols
WB protocol for GAPDH antibody 10494-1-APDownload protocol
IHC protocol for GAPDH antibody 10494-1-APDownload protocol
IF protocol for GAPDH antibody 10494-1-APDownload protocol
IP protocol for GAPDH antibody 10494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Nature

Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.

Authors - Xiao-Yi Lu
mouseWB

Nature

MAP3K2-regulated intestinal stromal cells define a distinct stem cell niche.

Authors - Ningbo Wu
humanWB

Nature

Altered TMPRSS2 usage by SARS-CoV-2 Omicron impacts tropism and fusogenicity.

Authors - Bo Meng
humanWB

Cell

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

Authors - Lei Sun
HUMANWB

Science

Vitamin C selectively kills KRAS and BRAF mutant colorectal cancer cells by targeting GAPDH.

Authors - Jihye Yun
mouseWB

Cell

Induction of pluripotency in mouse somatic cells with lineage specifiers.

Authors - Shu Jian J

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Verena (Verified Customer) (11-09-2022)

Gives a nice, strong and specific signal in Western Blot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Mouse
FH

Christine (Verified Customer) (10-27-2022)

Very good loading control antibody, strong signal when used at 1:15,000 over night, can probably be diluted much more.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:15,000
  • Cell Tissue Type: HEK293T
FH

Janelle (Verified Customer) (09-12-2022)

Perfect for WB!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
FH

Jake (Verified Customer) (11-30-2021)

GAPDH Ab works perfectly on western blot at low dilutions, will definitely buy again.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: BV-2 Cell Line
FH

Meg (Verified Customer) (02-25-2020)

It worked well for western blotting.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: THP-1
FH

Yuan (Verified Customer) (10-15-2019)

GAPDH is working very well on seminal plasma and blood plasma.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Human seminal plasma and blood plasma
FH

Hailey (Verified Customer) (10-07-2019)

This antibody is a fantastic loading control! I use this as a loading control for all my western blots where protein loading needs to be precise.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse testis
GAPDH Antibody Western Blot, validation (1:500 dilution) in Mouse testis (Cat no:10494-1-AP)
FH

Prasanth (Verified Customer) (08-19-2019)

The band is so clear, and very specific band with almost no background noise for GAPDH

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Fibroblasts
FH

Shannon (Verified Customer) (12-11-2018)

  • Applications: Cell culture,
  • Cell Tissue Type: rodent