HRP-conjugated GAPDH Monoclonal antibody

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  WB analysis of multi-cells/tissue using HRP-60004

  Western blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody HRP-60004 at a dilution of 1:10000.

• 

  WB analysis using HRP-60004

  Various lysates were subjected to SDS PAGE followed by western blot with HRP-60004 (GAPDH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using HRP-60004

  HeLa cells were subjected to SDS PAGE followed by western blot with HRP-60004 (GAPDH antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using HRP-60004

  HeLa cells were subjected to SDS PAGE followed by western blot with HRP-60004 (GAPDH antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

GAPDH Monoclonal Antibody for WB

Cat No. HRP-60004

Clone No.1E6D9

Publications(735)

Reviews (8)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, zebrafish, plant and More (7)

Applications

WB, IF, IP, CoIP, RIP

Synonyms

1E6D9

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, HepG2 cells, HEK-293 cells, mouse lung tissue, mouse liver tissue, mouse kidney tissue, mouse heart tissue, mouse thymus tissue, mouse spleen tissue. mouse skin tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 706 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Product Information

HRP-60004 targets GAPDH in WB, IF, IP, CoIP, RIP applications and shows reactivity with human, mouse, rat, zebrafish, plant samples.

• Tested Reactivity: human, mouse, rat, zebrafish, plant
• Cited Reactivity: human, mouse, rat, pig, rabbit, monkey, chicken, hamster, sheep, honey bees
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: glyceraldehyde-3-phosphate dehydrogenase
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC004109
• Gene Symbol: GAPDH
• Gene ID (NCBI): 2597
• RRID: AB_2737588
• Conjugate: HRP
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P04406
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Publications

Species	Application	Title
human	WB	Nature; DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25.; Authors - Linbin Yang
human	WB	Mol Cancer; Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer; Authors - Rashmi Bharti
human	WB	Nat Biotechnol; Direct targeting of amplified gene loci for proapoptotic anticancer therapy.; Authors - Meetu Kaushik Tiwari
human	WB	Cell; Programmable RNA-Guided RNA Effector Proteins Built from Human Parts.; Authors - Simone Rauch
human	WB	Cell; Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages.; Authors - Shicheng Su
human	WB	Cell; CD10+GPR77+Cancer-Associated Fibroblasts Promote Cancer Formation and Chemoresistance by Sustaining Cancer Stemness.; Authors - Shicheng Su

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Moritz (Verified Customer) (09-12-2024)

The antibody worked very well at a dilution of 1:10k in WB using HEL293T cell lysate as a loading control.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: HEK293T

HRP GAPDH Antibody Western Blot validation (1:10000 dilution) in HEK293T (Cat no:HRP-60004)

FH

Julia (Verified Customer) (08-07-2024)

Really good antibody with specific band at the correct size. Conjugation with HRP allows quickworks great to get results quickly.

• Applications: Western Blot
• Primary Antibody Dilution: 1:15000
• Cell Tissue Type: mESC

HRP GAPDH Antibody Western Blot validation (1:15000 dilution) in mESC (Cat no:HRP-60004)

FH

T (Verified Customer) (03-20-2024)

Worked great with multiple tissue types. Highly recommend!

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000

FH

Tsimafei (Verified Customer) (07-15-2022)

30µg of total protein were loaded on the gel. Antibodies were incubated ON at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mESCs

HRP GAPDH Antibody Western Blot validation (1:5000 dilution) in mESCs (Cat no:HRP-60004)

FH

Hala (Verified Customer) (02-25-2022)

it works very good but i got an aspecific band around 30KDa

• Applications: Western Blot
• Primary Antibody Dilution: 1/15000
• Cell Tissue Type: hepG2

HRP GAPDH Antibody Western Blot validation (1/15000 dilution) in hepG2 (Cat no:HRP-60004)

FH

Laura (Verified Customer) (01-10-2020)

Works well

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293T

FH

Benjamin (Verified Customer) (01-07-2020)

Works really well with high sensitivity and removes the need for a secondary. Needs to be dilute as GAPDH bands will be too intense at higher dilutions.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:25000
• Cell Tissue Type: HEK293T, SHSY5Y

FH

Kathryn (Verified Customer) (12-10-2019)

Used at 1:5000 in 5% BSA on a small amount of neonatal mouse ovary and worked beautifully.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mouse ovary

HRP GAPDH Antibody Western Blot, validation (1:5000 dilution) in mouse ovary (Cat no:HRP-60004)