HRP-conjugated GAPDH Monoclonal antibody

GAPDH Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, zebrafish, plant and More (2)

Applications

WB, ELISA

Conjugate

HRP Fluorescent Dye

Cat no : HRP-60004

Synonyms

G3PD, GAPD, GAPDH, HRP-G3PD, HRP-GAPD, HRP-GAPDH, HRP-OK/SW cl.12, OK/SW cl.12



Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 159 publications below

Product Information

HRP-60004 targets GAPDH in WB, ELISA applications and shows reactivity with human, mouse, rat, zebrafish, plant samples.

Tested Reactivity human, mouse, rat, zebrafish, plant
Cited Reactivity hamster, human, mouse, rat, swine
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Recombinant Protein
Full Name glyceraldehyde-3-phosphate dehydrogenase
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC004109
Gene symbol GAPDH
Gene ID (NCBI) 2597
Conjugate HRP Fluorescent Dye
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Protocols

Product Specific Protocols
WB protocol for HRP-conjugated GAPDH antibody HRP-60004Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

PLoS Genet

β-actin regulates a heterochromatin landscape essential for optimal induction of neuronal programs during direct reprograming.

Authors - Xin Xie
humanWB

J Cell Mol Med

MiR-552 promotes the proliferation, migration and EMT of hepatocellular carcinoma cells by inhibiting AJAP1 expression.

Authors - Weiqing Qu
mouseWB

Sci Adv

MRGPR-mediated activation of local mast cells clears cutaneous bacterial infection and protects against reinfection.

Authors - Mohammad Arifuzzaman
humanWB

Cancers (Basel)

Transfection with GLS2 Glutaminase (GAB) Sensitizes Human Glioblastoma Cell Lines to Oxidative Stress by a Common Mechanism Involving Suppression of the PI3K/AKT Pathway.

Authors - Ewelina Majewska
mouseWB

Nature

Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells.

Authors - Dali Han
humanWB

Mol Med Rep

BP‑1‑102 exerts an antitumor effect on the AGS human gastric cancer cell line through modulating the STAT3 and MAPK signaling pathways.

Authors - Xiaoxia Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Laura (Verified Customer) (01-10-2020)

Works well

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK293T
FH

Benjamin (Verified Customer) (01-07-2020)

Works really well with high sensitivity and removes the need for a secondary. Needs to be dilute as GAPDH bands will be too intense at higher dilutions.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:25000
  • Cell Tissue Type: HEK293T, SHSY5Y
FH

Kathryn (Verified Customer) (12-10-2019)

Used at 1:5000 in 5% BSA on a small amount of neonatal mouse ovary and worked beautifully.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: mouse ovary
HRP-conjugated GAPDH Antibody Western Blot, validation (1:5000 dilution) in mouse ovary (Cat no:HRP-60004)