|Positive WB detected in||HeLa cells, HEK-293 cells, Jurkat cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Sample-dependent, check data in validation data gallery|
CL488-60004 targets GAPDH in WB applications and shows reactivity with human, mouse, rat, zebrafish, yeast, plant samples.
|Tested Reactivity||human, mouse, rat, zebrafish, yeast, plant|
|Cited Reactivity||human, rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||GAPDH fusion protein Ag0766|
|Full Name||glyceraldehyde-3-phosphate dehydrogenase|
|Calculated molecular weight||36 kDa|
|Observed molecular weight||36 kDa|
|GenBank accession number||BC004109|
|Gene ID (NCBI)||2597|
|Conjugate||CoraLite®488 Fluorescent Dye|
|Excitation/Emission maxima wavelengths||488 nm/515 nm|
|Purification Method||Protein A purification|
|Storage Buffer||PBS buffer with 50% glycerol, pH7.3.|
|Storage Conditions||Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
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