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GAPDH Antibody 606 Publications

Mouse Monoclonal| Catalog number: 60004-1-Ig |CloneNo.: 1E6D9

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Con: 150 μg/150 μl

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Tested Applications
Applications:
ELISA, FC, IF, IHC, IP, WB
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Images See all

60004-1-Ig;HepG2 cells

Western blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody 60004-1-Ig at a dilution of 1:10000.

60004-1-Ig;HeLa cells

Western blot of Hela cell with anti-GAPDH (60004-1-Ig) at various dilutions.

60004-1-Ig;HeLa Cells

IP result of anti-GAPDH (60004-1-Ig for IP and Detection) with HeLa cell lysate.

60004-1-Ig;arabidopsis whole plant tissue

arabidopsis, rice, wheat, corn whole plant tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig( GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours

60004-1-Ig;human kidney tissue

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 60004-1-Ig( GAPDH Antibody) at dilution of 1:50 (under 10x lens)

60004-1-Ig;human kidney tissue

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 60004-1-Ig( GAPDH Antibody) at dilution of 1:50 (under 40x lens)

60004-1-Ig;Ethacrynic acid treated HeLa cells

Immunofluorescent analysis of Ethacrynic acid treated HeLa cells using 60004-1-Ig(GAPDH antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IgG (red).

60004-1-Ig;HeLa cells

1X10^6 HeLa cells were stained with 0.2ug GAPDH antibody (60004-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IgG with dilution 1:100.

Species specificity:
human, mouse, rat, zebrafish, yeast, plant, cow, Cyprinus carpio, Eelworm, hamster, monkey, moth, pig, Xenopus laevis
Positive WB detected in:
HeLa cells, arabidopsis whole plant tissue, Raji cells, HEK-293 cells, HepG2 cells, soybean whole plant tissue, whole Nematode tissue, zebrafish tissue, whole yeast cells
Positive IP detected in:
HeLa Cells
Positive IHC detected in:
human kidney tissue, human breast cancer tissue, human kidney tissue
Positive IF detected in:
Ethacrynic acid treated HeLa cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:2000-1:16000
IP : 0.5-4.0 ug for IP and 1:2000-1:12000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
GAPDH fusion protein Ag0766
Full name:
glyceraldehyde-3-phosphate dehydrogenase
Calculated molecular weight:
36 kDa
Observed molecular weight:
36 kDa
GenBank accession number:
BC004109
Gene ID (NCBI):
2597
Gene symbol
GAPDH
Synonyms
G3PD, GAPD, GAPDH, OK/SW cl.12
Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation. This antibody is a mouse monoclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.


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