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GAPDH Antibody 1034 Publications

Mouse Monoclonal| Catalog number: 60004-1-Ig |CloneNo.: 1E6D9

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Reviews(2)

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

GAPDH Antibody WB HepG2 cells 60004-1-Ig

Western blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody 60004-1-Ig at a dilution of 1:10000.

GAPDH Antibody WB HeLa cells 60004-1-Ig

Western blot of Hela cell with anti-GAPDH (60004-1-Ig) at various dilutions.

GAPDH Antibody IP HeLa Cells 60004-1-Ig

IP result of anti-GAPDH (60004-1-Ig for IP and Detection) with HeLa cell lysate.

GAPDH Antibody WB arabidopsis whole plant tissue 60004-1-Ig

arabidopsis, rice, wheat, corn whole plant tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig( GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours

GAPDH Antibody IHC human kidney tissue 60004-1-Ig

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 60004-1-Ig( GAPDH Antibody) at dilution of 1:50 (under 10x lens)

GAPDH Antibody IHC human kidney tissue 60004-1-Ig

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 60004-1-Ig( GAPDH Antibody) at dilution of 1:50 (under 40x lens)

GAPDH Antibody IF Ethacrynic acid treated HeLa cells 60004-1-Ig

Immunofluorescent analysis of Ethacrynic acid treated HeLa cells using 60004-1-Ig(GAPDH antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IgG (red).

GAPDH Antibody FC HeLa cells 60004-1-Ig

1X10^6 HeLa cells were stained with 0.2ug GAPDH antibody (60004-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IgG with dilution 1:100.

Species specificity:
human, mouse, rat, zebrafish, yeast, plant, beagle, chicken, cow, Cyprinus carpio, deer, Eelworm, Hamster, monkey, pig, sheep
Positive WB detected in:
HeLa cells, arabidopsis whole plant tissue, Raji cells, HEK-293 cells, HepG2 cells, soybean whole plant tissue, whole Nematode tissue, zebrafish tissue, whole yeast cells
Positive IP detected in:
HeLa Cells
Positive IHC detected in:
human kidney tissue, human breast cancer tissue
Positive IF detected in:
Ethacrynic acid treated HeLa cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:2000-1:12000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
GAPDH fusion protein Ag0766
Full name:
glyceraldehyde-3-phosphate dehydrogenase
Calculated molecular weight:
36 kDa
Observed molecular weight:
36 kDa
GenBank accession number:
BC004109
Gene ID (NCBI):
2597
Gene symbol
GAPDH
Synonyms
G3PD, GAPD, GAPDH, OK/SW cl.12

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Customer Reviews

Share your scientific insight on this antibody

GAPDH Mouse Monoclonal antibody

Qunfeng Wu, 01-11-2018
Applications
Western Blot,
Lot Number
10004129
Primary Antibody Dilution
1:10000
Secondary Antibody Dilution
1:10000
Cell/Tissue Type
Huh7 cells

GAPDH Mouse Monoclonal antibody

Sinu John, 01-10-2018
Applications
Western Blot,
Lot Number
1
Primary Antibody Dilution
1:1000
Secondary Antibody Dilution
1:5000
Cell/Tissue Type
Human THP1

Worked well in the western blot

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