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GAPDH Antibody 1391 Publications

Mouse Monoclonal| Catalog number: 60004-1-Ig |CloneNo.: 1E6D9

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18 customer reviews

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

GAPDH Antibody WB HepG2 cells 60004-1-Ig

Western blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody 60004-1-Ig at a dilution of 1:10000.

GAPDH Antibody WB HeLa cells 60004-1-Ig

Western blot of Hela cell with anti-GAPDH (60004-1-Ig) at various dilutions.

GAPDH Antibody IP HeLa Cells 60004-1-Ig

IP result of anti-GAPDH (60004-1-Ig for IP and Detection) with HeLa cell lysate.

GAPDH Antibody WB arabidopsis whole plant tissue 60004-1-Ig

arabidopsis, rice, wheat, corn whole plant tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig( GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours

GAPDH Antibody IHC human kidney tissue 60004-1-Ig

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60004-1-Ig( GAPDH Antibody) at dilution of 1:50 (under 10x lens)

GAPDH Antibody IHC human kidney tissue 60004-1-Ig

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60004-1-Ig( GAPDH Antibody) at dilution of 1:50 (under 40x lens)

GAPDH Antibody IF Ethacrynic acid treated HeLa cells 60004-1-Ig

Immunofluorescent analysis of Ethacrynic acid treated HeLa cells using 60004-1-Ig(GAPDH antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IgG (red).

GAPDH Antibody FC HeLa cells 60004-1-Ig

1X10^6 HeLa cells were stained with 0.2ug GAPDH antibody (60004-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IgG with dilution 1:100.

Species specificity:
human, mouse, rat, zebrafish, yeast, plant, beagle, chicken, cow, Cynomorium songaricum Rupr, Cyprinus carpio, deer, dog, Eelworm, Hamster, hamsters
Positive WB detected in:
HeLa cells, HepG2 cells, zebrafish tissue, Raji cells, HEK-293 cells, whole Nematode tissue, soybean whole plant tissue, arabidopsis whole plant tissue, whole yeast cells
Positive IP detected in:
HeLa Cells
Positive IHC detected in:
human kidney tissue, human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
Ethacrynic acid treated HeLa cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:2000-1:12000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
GAPDH fusion protein Ag0766
Full name:
glyceraldehyde-3-phosphate dehydrogenase
Calculated molecular weight:
36 kDa
Observed molecular weight:
36 kDa
GenBank accession number:
BC004109
Gene ID (NCBI):
2597
Gene symbol
GAPDH
Synonyms
G3PD, GAPD, GAPDH, OK/SW cl.12

Background

What is the molecular weight of GAPDH?

The molecular weight of GAPDH is 36 kD.


What is GAPDH?

GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase and is often referred to as a “housekeeping” 

protein. It is recognized as an important enzyme involved in metabolic pathways, aiding in the glycolytic 

production of ATP and pyruvate (PMID: 21895736).


What is the function of GAPDH?

GAPDH is responsible for catalyzing the phosphorylation of glyceraldehyde-3-phosphate into D-glycerate-1,3-biphosphate. While this function of GAPDH is considered its primary function, GAPDH is also involved, even 

critical, in other cell functions, some of which are still being studied. Recent studies have suggested that GAPDH 

is a key factor in regulating apoptosis, translocating to the nucleus under certain stressful conditions and acting 

as a signaling factor in oxidative stress-induced apoptosis. Other studies indicate that GAPDH plays a role in 

transporting vesicles from the endoplasmic reticulum to the Golgi body (PMIDs: 21895736, 12829261, 25859407, 

11035021).


Where in the cell is GAPDH localized?

GAPDH is expressed primarily in the cytosolic and membrane regions, with minimal expression in the nucleus 

(PMIDs: 21895736, 12829261).


Is there a reason to use an antibody targeting GAPDH if my research is not concerned with glycolysis or nuclear 

functions?

Yes! Because GAPDH is often highly expressed throughout different tissues and cell types, GAPDH expression is 

useful as a control for Western blots and RT-PCR.


What are the known protein-protein interactions of GAPDH?

GAPDH interacts with other proteins to regulate nuclear translocation and GAPDH catalytic activity. Some of 

these proteins include TPPP, SIAH1, CHP1, RILPL1/GOSPEL and FKBP6 (PMIDs: 21895736, 25859407).


Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

GAPDH Mouse Monoclonal antibody

Praveen (Verified Customer), 04-15-2019
Applications
Western Blot,
GAPDH Antibody Western Blot, validation ( dilution) in Ac16 (Cat no:60004-1-Ig)

GAPDH Antibody Western Blot, validation ( dilution) in Ac16 (Cat no:60004-1-Ig)

Cell/Tissue Type
Ac16
Lot Number
Good

GAPDH Mouse Monoclonal antibody

Vanessa (Verified Customer), 04-14-2019
Applications
Western Blot,
Primary Antibody Dilution
1:5000
No image submitted with review

No image submitted with review

Cell/Tissue Type
brain
Lot Number
Total cell lysate (5-20 ug) was resolved on 4-12% Bis-Tris gels and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% BSA in PBS/0.1% Tween-20) for 1h. Membrane was incubated with anti-GAPDH in blocking buffer (1:5000) at 4C overnight. After washing, membrane was incubated in anti-mouse-HRP in blocking bufffer (1:10000) for 2h at room temperature. Membrane was developed in ECF reagent and imaged on a chemiluminescence detection system.

GAPDH Mouse Monoclonal antibody

Damaris (Verified Customer), 04-03-2019
Applications
Western Blot,
Primary Antibody Dilution
1:1000
GAPDH Antibody Western Blot, validation (1:1000 dilution) in brain lysate (Cat no:60004-1-Ig)

GAPDH Antibody Western Blot, validation (1:1000 dilution) in brain lysate (Cat no:60004-1-Ig)

Cell/Tissue Type
brain lysate
Lot Number
Excellent antibody! It gives clean and specific results

GAPDH Mouse Monoclonal antibody

JIMMY (Verified Customer), 03-26-2019
Applications
Western Blot,
No image submitted with review

No image submitted with review

Lot Number
This antibody offers a good detection of GAPDH on mouse cells such as macrophages and microglia through western blots.

GAPDH Mouse Monoclonal antibody

Elisabeth (Verified Customer), 02-05-2019
Applications
Western Blot,
Primary Antibody Dilution
10,000
GAPDH Antibody Western Blot, validation (10,000 dilution) in HeLa cells lysate (Cat no:60004-1-Ig)

GAPDH Antibody Western Blot, validation (10,000 dilution) in HeLa cells lysate (Cat no:60004-1-Ig)

Cell/Tissue Type
HeLa cells lysate
Lot Number
Total cell lysate (15 ug) was resolved on 4-12% Bis-Tris gels and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk in PBS/0.1% Tween-20) for 1h. Membrane was incubated with anti-GAPDH in blocking buffer (1:10,000) at 4C overnight. After washing, membrane was incubated in anti-mouse-HRP in blocking bufffer (1:5000) for 2h at room temperature. Membrane was developed in ECL reagent and imaged on a chemiluminescence detection system.

GAPDH Mouse Monoclonal antibody

Stephen (Verified Customer), 01-31-2019
Applications
Western Blot,
Primary Antibody Dilution
1:20000
GAPDH Antibody Western Blot, validation (1:20000 dilution) in HeLa (Cat no:60004-1-Ig)

GAPDH Antibody Western Blot, validation (1:20000 dilution) in HeLa (Cat no:60004-1-Ig)

Cell/Tissue Type
HeLa
Lot Number
great antibody for WB as a loading control used 1:20000

GAPDH Mouse Monoclonal antibody

Kishor (Verified Customer), 01-30-2019
Applications
Western Blot,
Primary Antibody Dilution
1:5000
GAPDH Antibody Western Blot, validation (1:5000 dilution) in H69, HUCCT-1 (Cat no:60004-1-Ig)

GAPDH Antibody Western Blot, validation (1:5000 dilution) in H69, HUCCT-1 (Cat no:60004-1-Ig)

Cell/Tissue Type
H69, HUCCT-1
Lot Number
I working good for Western Blotting (1:5000)

GAPDH Mouse Monoclonal antibody

Wei (Verified Customer), 12-04-2018
Applications
Western Blot,
Primary Antibody Dilution
1:1000
No image submitted with review

No image submitted with review

Cell/Tissue Type
C2C12
Lot Number
In muscle cells, GAPDH antibody shows two bands while in cancer cells there is only one band
Proteintech Response
Thank you for your review. One possible reason for your muscle cell result is post-translational modification of GAPDH (https://www.uniprot.org/uniprot/P04406#ptm_processing).

GAPDH Mouse Monoclonal antibody

Robert (Verified Customer), 12-03-2018
Applications
Western Blot,
Primary Antibody Dilution
1:10000
No image submitted with review

No image submitted with review

Cell/Tissue Type
neuronal cell culture
Lot Number
Strong and clean signal in WB

GAPDH Mouse Monoclonal antibody

Guillaume (Verified Customer), 11-21-2018
Applications
Western Blot,
Primary Antibody Dilution
1/1000
GAPDH Antibody Western Blot, validation (1/1000 dilution) in Prostate cancer cells (Cat no:60004-1-Ig)

GAPDH Antibody Western Blot, validation (1/1000 dilution) in Prostate cancer cells (Cat no:60004-1-Ig)

Cell/Tissue Type
Prostate cancer cells
Lot Number
This antibody is useful as a cytoplasmic control for cyto-nuclear extractions.

GAPDH Mouse Monoclonal antibody

Ruth (Verified Customer), 11-21-2018
Applications
Western Blot,
Primary Antibody Dilution
1:40000
No image submitted with review

No image submitted with review

Cell/Tissue Type
Human cell line lysates
Lot Number

GAPDH Mouse Monoclonal antibody

Tingting (Verified Customer), 11-20-2018
Applications
Western Blot,
Primary Antibody Dilution
1:1000
No image submitted with review

No image submitted with review

Cell/Tissue Type
Trophoblast
Lot Number
The bands of the GADPH looked very good! The antibody used in trophoblast cell and diluted in 1:1000.

GAPDH Mouse Monoclonal antibody

marian (Verified Customer), 11-20-2018
Applications
Western Blot,
Primary Antibody Dilution
1:1000
GAPDH Antibody Western Blot, validation (1:1000 dilution) in colon (Cat no:60004-1-Ig)

GAPDH Antibody Western Blot, validation (1:1000 dilution) in colon (Cat no:60004-1-Ig)

Cell/Tissue Type
colon
Lot Number

GAPDH Mouse Monoclonal antibody

vanessa (Verified Customer), 11-20-2018
Applications
Cell culture,
No image submitted with review

No image submitted with review

Lot Number
very efficient product and great customer service

GAPDH Mouse Monoclonal antibody

Vermont (Verified Customer), 11-20-2018
Applications
Western Blot,
Primary Antibody Dilution
1:5000
GAPDH Antibody Western Blot, validation (1:5000 dilution) in macrophage (Cat no:60004-1-Ig)

GAPDH Antibody Western Blot, validation (1:5000 dilution) in macrophage (Cat no:60004-1-Ig)

Cell/Tissue Type
macrophage
Lot Number
great sensitivity and reproducibility

GAPDH Mouse Monoclonal antibody

Saswati (Verified Customer), 11-20-2018
Applications
Western Blot,
Primary Antibody Dilution
1:8000
No image submitted with review

No image submitted with review

Cell/Tissue Type
Human breast cancer
Lot Number

GAPDH Mouse Monoclonal antibody

Qunfeng (Verified Customer), 01-11-2018
Applications
Western Blot,
Primary Antibody Dilution
1:10000
Secondary Antibody Dilution
1:10000
No image submitted with review

No image submitted with review

Cell/Tissue Type
Huh7 cells
Lot Number
10004129

GAPDH Mouse Monoclonal antibody

Sinu (Verified Customer), 01-10-2018
Applications
Western Blot,
Primary Antibody Dilution
1:1000
Secondary Antibody Dilution
1:5000
No image submitted with review

No image submitted with review

Cell/Tissue Type
Human THP1
Lot Number
1
Worked well in the western blot

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