GAPDH Monoclonal antibody

GAPDH Monoclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, yeast, plant and More (10)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1E6D9

Cat no : 60004-1-Ig

Synonyms

G3PD, GAPD, GAPDH, OK/SW cl.12



Tested Applications

Positive WB detected inHeLa cells, soybean whole plant tissue, arabidopsis whole plant tissue, HepG2 cells, ROS1728 cells, pig brain tissue, zebrafish tissue, whole yeast cells, whole Nematode tissue, HEK-293 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, C2C12 cells, SP2/0 cells, rat brain tissue, mouse brain tissue
Positive IP detected inHeLa Cells
Positive IF detected inEthacrynic acid treated HeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:2000-1:12000 for WB
Immunofluorescence (IF)IF : 1:200-1:2000
Sample-dependent, check data in validation data gallery

Product Information

60004-1-Ig targets GAPDH in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, yeast, plant samples.

Tested Reactivity human, mouse, rat, yeast, plant
Cited Reactivity A. flavus, azalea R. hainanense, beagle, Bovine, canine, carp, chicken, cow, Cynomorium songaricum Rupr, cyprinid
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GAPDH fusion protein Ag0766
Full Name glyceraldehyde-3-phosphate dehydrogenase
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC004109
Gene symbol GAPDH
Gene ID (NCBI) 2597
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

What is the molecular weight of GAPDH?

The molecular weight of GAPDH is 36 kD.


What is GAPDH?

GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase and is often referred to as a “housekeeping” 

protein. It is recognized as an important enzyme involved in metabolic pathways, aiding in the glycolytic 

production of ATP and pyruvate (PMID: 21895736).


What is the function of GAPDH?

GAPDH is responsible for catalyzing the phosphorylation of glyceraldehyde-3-phosphate into D-glycerate-1,3-biphosphate. While this function of GAPDH is considered its primary function, GAPDH is also involved, even 

critical, in other cell functions, some of which are still being studied. Recent studies have suggested that GAPDH 

is a key factor in regulating apoptosis, translocating to the nucleus under certain stressful conditions and acting 

as a signaling factor in oxidative stress-induced apoptosis. Other studies indicate that GAPDH plays a role in 

transporting vesicles from the endoplasmic reticulum to the Golgi body (PMIDs: 21895736, 12829261, 25859407, 

11035021).


Where in the cell is GAPDH localized?

GAPDH is expressed primarily in the cytosolic and membrane regions, with minimal expression in the nucleus 

(PMIDs: 21895736, 12829261).


Is there a reason to use an antibody targeting GAPDH if my research is not concerned with glycolysis or nuclear 

functions?

Yes! Because GAPDH is often highly expressed throughout different tissues and cell types, GAPDH expression is 

useful as a control for Western blots and RT-PCR.


What are the known protein-protein interactions of GAPDH?

GAPDH interacts with other proteins to regulate nuclear translocation and GAPDH catalytic activity. Some of 

these proteins include TPPP, SIAH1, CHP1, RILPL1/GOSPEL and FKBP6 (PMIDs: 21895736, 25859407).


Note: For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable.


Protocols

Product Specific Protocols
WB protocol for GAPDH antibody 60004-1-IgDownload protocol
IF protocol for GAPDH antibody 60004-1-IgDownload protocol
FC protocol for GAPDH antibody 60004-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

BMB Rep

Overexpression of CXCL2 inhibits cell proliferation and promotes apoptosis in hepatocellular carcinoma.

Authors - Jun Ding
ratWB

J Cell Mol Med

Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity.

Authors - Yi Guan
mouseWB

FASEB J

Expression patterns of C1ql4 and its cell-adhesion GPCR Bai3 in the murine testis and functional roles in steroidogenesis.

Authors - Anni Tan
humanWB

Free Radic Biol Med

5-(3,4-Difluorophenyl)-3-(6-methylpyridin-3-yl)-1,2,4-oxadiazole (DDO-7263), a Novel Nrf2 Activator Targeting Brain Tissue, Protects Against MPTP-induced Subacute Parkinson's Disease in Mice by Inhibiting the NLRP3 Inflammasome and Protects PC12 Cells Against Oxidative Stress.

Authors - Li-Li Xu
hamsterWB

Metab Eng

Continuous Translation of Circularized mRNA Improves Recombinant Protein Titer.

Authors - Alan Costello
ratWB

Front Physiol

Sesamin Protects the Femoral Head From Osteonecrosis by Inhibiting ROS-Induced Osteoblast Apoptosis in Rat Model.

Authors - Shuang Deng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Eiko (Verified Customer) (10-16-2020)

This antibody worked quite well in my western blotting. I used 5% skim milk TBS-T for blocking and antibody dilution.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: hTERT-RPE1
GAPDH Antibody Western Blot validation (1:2000 dilution) in hTERT-RPE1 (Cat no:60004-1-Ig)
FH

Tom (Verified Customer) (09-28-2020)

HEK293T lysate (10ug) ran on 4-20% gradient gel. Blocking in 5% BSA for 1 hour, followed by HSP27 (1:5000 in block) overnight at 4 degrees. Goat anti-mouse HRP (1:10,000) for 1 hour in block.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK293T
GAPDH Antibody Western Blot validation (1:5000 dilution) in HEK293T (Cat no:60004-1-Ig)
FH

Federica (Verified Customer) (06-08-2020)

Excellent antibody. I used in WB on HeLa lysate and the results was extremely clear. No unspecific bands detected. Perfect as loading control.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: HeLa cells
GAPDH Antibody Western Blot, validation (1:10000 dilution) in HeLa cells (Cat no:60004-1-Ig)
FH

Alinda (Verified Customer) (01-27-2020)

Good antibody for Western BLOT. antibody diluted in 5%milk.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK 293T
GAPDH Antibody Western Blot, validation (1:1000 dilution) in HEK 293T (Cat no:60004-1-Ig)
FH

Aamir (Verified Customer) (01-19-2020)

Good signal for WB

  • Applications: Western Blot,
  • Cell Tissue Type: HEKs
FH

David (Verified Customer) (01-13-2020)

Excellent antibody. Single band at the correct molecular weight. No background and can be used at high dilutions.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: SH-SY5Y
FH

saswati (Verified Customer) (01-08-2020)

Need a 1 hr block with 5% milk / TBST as usual. 1 hr room temp with the same blocking media with a 1:8000 dilution, followed by secondary anti mouse ( same dilution) , HRP conjugated 1 hr room temp. Detect and document. 4 is rating , since a 1:8000 fold is tad bit low, have used better dilution ( 1:100,000) in the past, however that product is not available these days

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1 to 8000
  • Cell Tissue Type: MDA-MB-231
FH

Emma (Verified Customer) (12-22-2019)

This antibody works well detecting GAPDH in 3t3-L1 and primary mouse embryonic fibroblasts.

  • Applications: Western Blot,
  • Cell Tissue Type: 3t3-L1, pMEF
FH

Nhu Quynh (Verified Customer) (12-16-2019)

High afffinity and specific antibody. Useful for protein controls in western blots.

  • Applications: Western Blot,
  • Cell Tissue Type: Cardiomyocytes
FH

Jia (Verified Customer) (11-12-2019)

This antibody works really well.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: CHO-K1 cell
GAPDH Antibody Western Blot, validation (1:1000 dilution) in CHO-K1 cell (Cat no:60004-1-Ig)
FH

Juan (Verified Customer) (10-20-2019)

WB, it works well in 1:10000 dilution.

  • Applications: Western Blot,
FH

Iram (Verified Customer) (10-01-2019)

Working excellent

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Pancreatic and Colon cancer cells
GAPDH Antibody Western Blot, validation (1:5000 dilution) in Pancreatic and Colon cancer cells (Cat no:60004-1-Ig)
FH

RICHA (Verified Customer) (09-25-2019)

Excellent antibody. Clean and specific

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:25000
  • Cell Tissue Type: Protein from mouse tail tendon
GAPDH Antibody Western Blot, validation (1:25000 dilution) in Protein from mouse tail tendon (Cat no:60004-1-Ig)
FH

Pau (Verified Customer) (09-02-2019)

Excellent and clean Western blots.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: HEK293T
FH

Tanusree (Verified Customer) (08-14-2019)

The antibody works good in mouse brain tissue in western blotting.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain
FH

Praveen (Verified Customer) (04-15-2019)

Good

  • Applications: Western Blot,
  • Cell Tissue Type: Ac16
GAPDH Antibody Western Blot, validation ( dilution) in Ac16 (Cat no:60004-1-Ig)
FH

Shubham (Verified Customer) (04-15-2019)

Very good

  • Applications: Western Blot,
  • Cell Tissue Type: AC16
GAPDH Antibody Western Blot, validation ( dilution) in AC16 (Cat no:60004-1-Ig)
FH

Vanessa (Verified Customer) (04-14-2019)

Total cell lysate (5-20 ug) was resolved on 4-12% Bis-Tris gels and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% BSA in PBS/0.1% Tween-20) for 1h. Membrane was incubated with anti-GAPDH in blocking buffer (1:5000) at 4C overnight. After washing, membrane was incubated in anti-mouse-HRP in blocking bufffer (1:10000) for 2h at room temperature. Membrane was developed in ECF reagent and imaged on a chemiluminescence detection system.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: brain
FH

Damaris (Verified Customer) (04-03-2019)

Excellent antibody! It gives clean and specific results

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: brain lysate
GAPDH Antibody Western Blot, validation (1:1000 dilution) in brain lysate (Cat no:60004-1-Ig)
FH

JIMMY (Verified Customer) (03-26-2019)

This antibody offers a good detection of GAPDH on mouse cells such as macrophages and microglia through western blots.

  • Applications: Western Blot,
FH

Elisabeth (Verified Customer) (02-05-2019)

Total cell lysate (15 ug) was resolved on 4-12% Bis-Tris gels and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk in PBS/0.1% Tween-20) for 1h. Membrane was incubated with anti-GAPDH in blocking buffer (1:10,000) at 4C overnight. After washing, membrane was incubated in anti-mouse-HRP in blocking bufffer (1:5000) for 2h at room temperature. Membrane was developed in ECL reagent and imaged on a chemiluminescence detection system.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 10,000
  • Cell Tissue Type: HeLa cells lysate
GAPDH Antibody Western Blot, validation (10,000 dilution) in HeLa cells lysate (Cat no:60004-1-Ig)
FH

Stephen (Verified Customer) (01-31-2019)

great antibody for WB as a loading control used 1:20000

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:20000
  • Cell Tissue Type: HeLa
GAPDH Antibody Western Blot, validation (1:20000 dilution) in HeLa (Cat no:60004-1-Ig)
FH

Kishor (Verified Customer) (01-30-2019)

I working good for Western Blotting (1:5000)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: H69, HUCCT-1
GAPDH Antibody Western Blot, validation (1:5000 dilution) in H69, HUCCT-1 (Cat no:60004-1-Ig)
FH

Wei (Verified Customer) (12-04-2018)

In muscle cells, GAPDH antibody shows two bands while in cancer cells there is only one band

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: C2C12
FH

Robert (Verified Customer) (12-03-2018)

Strong and clean signal in WB

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: neuronal cell culture
FH

Guillaume (Verified Customer) (11-21-2018)

This antibody is useful as a cytoplasmic control for cyto-nuclear extractions.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Prostate cancer cells
GAPDH Antibody Western Blot, validation (1/1000 dilution) in Prostate cancer cells (Cat no:60004-1-Ig)
FH

Ruth (Verified Customer) (11-21-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:40000
  • Cell Tissue Type: Human cell line lysates
FH

Tingting (Verified Customer) (11-20-2018)

The bands of the GADPH looked very good! The antibody used in trophoblast cell and diluted in 1:1000.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Trophoblast
FH

marian (Verified Customer) (11-20-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: colon
GAPDH Antibody Western Blot, validation (1:1000 dilution) in colon (Cat no:60004-1-Ig)
FH

vanessa (Verified Customer) (11-20-2018)

very efficient product and great customer service

  • Applications: Cell culture,
FH

Vermont (Verified Customer) (11-20-2018)

great sensitivity and reproducibility

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: macrophage
GAPDH Antibody Western Blot, validation (1:5000 dilution) in macrophage (Cat no:60004-1-Ig)
FH

Saswati (Verified Customer) (11-20-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:8000
  • Cell Tissue Type: Human breast cancer
FH

Qunfeng (Verified Customer) (01-11-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: Huh7 cells
FH

Sinu (Verified Customer) (01-10-2018)

Worked well in the western blot

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human THP1