GAPDH Monoclonal antibody

GAPDH Monoclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, yeast, plant, zebrafish and More (10)

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

6944

CloneNo.

1E6D9

Reviews (48)

Cat no : 60004-1-Ig

Synonyms

G3PD, GAPD, GAPDH, OK/SW cl.12

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Validation Data Gallery

• 

  WB analysis using 60004-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60004-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60004-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 60004-1-Ig

  Western blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody 60004-1-Ig at a dilution of 1:10000.

• 

  WB analysis of arabidopsis whole plant using 60004-1-Ig

  arabidopsis, rice, wheat, corn whole plant tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60004-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of whole Nematode using 60004-1-Ig

  whole Nematode tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of soybean whole plant using 60004-1-Ig

  soybean whole plant tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of zebrafish using 60004-1-Ig

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of whole yeast using 60004-1-Ig

  whole yeast cells were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 60004-1-Ig

  Western blot of Hela cell with anti-GAPDH (60004-1-Ig) at various dilutions.

• 

  IP experiment of HeLa Cells using 60004-1-Ig

  IP result of anti-GAPDH (60004-1-Ig for IP and Detection) with HeLa cell lysate.

• 

  IF Staining of HeLa using 60004-1-Ig

  Immunofluorescent analysis of EA treated HeLa cells using 60004-1-Ig(GAPDH antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IgG (red).

• 

  FC experiment of HeLa using 60004-1-Ig

  1X10^6 HeLa cells were stained with 0.2ug GAPDH antibody (60004-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IgG with dilution 1:100.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, ROS1728 cells, pig brain tissue, zebrafish tissue, whole yeast, whole Nematode tissue, soybean whole plant tissue, arabidopsis whole plant tissue, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, C6 cells, PC-12 cells, C2C12 cells, SP2/0 cells, rat brain tissue, mouse brain tissue
• Positive IP detected in: HeLa Cells
• Positive IF detected in: Ethacrynic acid treated HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:50000-1:500000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:2000-1:12000 for WB
• Immunofluorescence (IF): IF : 1:200-1:2000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 6651 publications below
• IHC: See 7 publications below
• IF: See 13 publications below
• IP: See 21 publications below
• FC: See 2 publications below
• CoIP: See 5 publications below
• ChIP: See 1 publications below

Product Information

60004-1-Ig targets GAPDH in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, yeast, plant, zebrafish samples.

• Tested Reactivity: human, mouse, rat, yeast, plant, zebrafish
• Cited Reactivity: Arabidopsis, Bombyx mori, bovine, Branchiostoma belcheri, brovine, Caenorhabditis elegans, canine, chicken, CHO cell line, Cynomorium songaricum
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GAPDH fusion protein Ag0766
• Full Name: glyceraldehyde-3-phosphate dehydrogenase
• Calculated molecular weight: 36 kDa
• Observed molecular weight: 36 kDa
• GenBank accession number: BC004109
• Gene symbol: GAPDH
• Gene ID (NCBI): 2597
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

What is the molecular weight of GAPDH?

The molecular weight of GAPDH is 36 kD.

What is GAPDH?

GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase and is often referred to as a "housekeeping" 

protein. It is recognized as an important enzyme involved in metabolic pathways, aiding in the glycolytic 

production of ATP and pyruvate (PMID: 21895736).

What is the function of GAPDH?

GAPDH is responsible for catalyzing the phosphorylation of glyceraldehyde-3-phosphate into D-glycerate-1,3-biphosphate. While this function of GAPDH is considered its primary function, GAPDH is also involved, even 

critical, in other cell functions, some of which are still being studied. Recent studies have suggested that GAPDH 

is a key factor in regulating apoptosis, translocating to the nucleus under certain stressful conditions and acting 

as a signaling factor in oxidative stress-induced apoptosis. Other studies indicate that GAPDH plays a role in 

transporting vesicles from the endoplasmic reticulum to the Golgi body (PMIDs: 21895736, 12829261, 25859407, 

11035021).

Where in the cell is GAPDH localized?

GAPDH is expressed primarily in the cytosolic and membrane regions, with minimal expression in the nucleus 

(PMIDs: 21895736, 12829261).

Is there a reason to use an antibody targeting GAPDH if my research is not concerned with glycolysis or nuclear 

functions?

Yes! Because GAPDH is often highly expressed throughout different tissues and cell types, GAPDH expression is 

useful as a control for Western blots and RT-PCR.

What are the known protein-protein interactions of GAPDH?

GAPDH interacts with other proteins to regulate nuclear translocation and GAPDH catalytic activity. Some of 

these proteins include TPPP, SIAH1, CHP1, RILPL1/GOSPEL and FKBP6 (PMIDs: 21895736, 25859407).

Note: For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable.

Publications

Species	Application	Title
human	WB	Nature; Allele-selective lowering of mutant HTT protein by HTT-LC3 linker compounds.; Authors - Zhaoyang Li
human	WB	Nature; Structure of LRRK2 in Parkinson's disease and model for microtubule interaction.; Authors - C K Deniston
human	WB	Nature; The molecular basis of tight nuclear tethering and inactivation of cGAS.; Authors - Baoyu Zhao
human,mouse	WB	Nature; Inhibition of PCSK9 potentiates immune checkpoint therapy for cancer.; Authors - Xinjian Liu
	WB	Nature; A phase-separated nuclear GBPL circuit controls immunity in plants.; Authors - Shuai Huang
human	WB	Nature; SARS-CoV-2 B.1.617.2 Delta variant replication and immune evasion.; Authors - Petra Mlcochova

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (09-27-2022)

The antibody was specific and detected a band close to 35 kD in BM-MSCs at 1:5000 dilution, overnight at 4 degrees.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Bone Marrow- MSCs

GAPDH Antibody Western Blot validation (1:5000 dilution) in Bone Marrow- MSCs (Cat no:60004-1-Ig)

FH

Janelle (Verified Customer) (09-12-2022)

Specific to target

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Yuki (Verified Customer) (08-23-2022)

A nice antibody to detect GAPDH in 293T cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:50000
• Cell Tissue Type: 293T

GAPDH Antibody Western Blot validation (1:50000 dilution) in 293T (Cat no:60004-1-Ig)

FH

Ralf (Verified Customer) (08-04-2022)

10µg of total mouse protein were loaded on the gel. Excellent antibodies!

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000

GAPDH Antibody Western Blot validation (1:3000 dilution) in (Cat no:60004-1-Ig)

FH

Tsimafei (Verified Customer) (07-15-2022)

30µg of total protein were loaded on the gel. Antibodies were incubated ON at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mESCs

GAPDH Antibody Western Blot validation (1:5000 dilution) in mESCs (Cat no:60004-1-Ig)

FH

Xin (Verified Customer) (04-15-2022)

A nice antibody to detect GAPDH in both mouse cortex lysates.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

GAPDH Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:60004-1-Ig)

FH

Shu Jun (Verified Customer) (04-14-2022)

Not applicable

• Applications: Western Blot
• Cell Tissue Type: HeLa cell

FH

Weihao (Verified Customer) (04-11-2022)

Great antibody! Clear band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000-10000
• Cell Tissue Type: mouse BMDM

FH

Charlotte (Verified Customer) (03-09-2022)

Membrane was blocked for 1 hour with 5% nonfat milk in TBS + 0.01% Tween, then probed overnight at 4C with a 1:20,000 dilution of a-GAPDH antibody in blocking buffer. After washing, the membrane was probed with an a-mouse secondary antibody at 1:50,000 in TBST. Under these conditions, the signal is very robust, even at a short exposure (~30 seconds) and has low background. The GAPDH antibody could likely be used at lower concentrations. Quite pleased with how it looks!

• Applications: Western Blot
• Primary Antibody Dilution: 1:20000
• Cell Tissue Type: Xenopus Embryo Extract

GAPDH Antibody Western Blot validation (1:20000 dilution) in Xenopus Embryo Extract (Cat no:60004-1-Ig)

FH

Uthra (Verified Customer) (03-03-2022)

Excellent antibody! It gives clean and specific results

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

GAPDH Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:60004-1-Ig)

FH

Jinwei (Verified Customer) (08-31-2021)

This GAPDH Monoclonal antibody Cat No. 60004-1-Ig works OK.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293

GAPDH Antibody Western Blot validation (1:5000 dilution) in HEK293 (Cat no:60004-1-Ig)

FH

IMRAN AKHTAR (Verified Customer) (04-15-2021)

This antibody is very good. We have used it many times and with really good results.

• Applications: Western Blot, Cell culture
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Ovarian cancer stem cells,

FH

Pradeep (Verified Customer) (02-02-2021)

Good antibody for WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: Mouse Heart

FH

Yin (Verified Customer) (01-27-2021)

293T lysate (20 ug) was loaded on 4-12% Bis-Tris gels and transferred to PVDF membrane. Membrane was incubated in 5% BSA in PBS/0.1% Tween-20 for 1h. Membrane was incubated with anti-GAPDH in blocking buffer (1:5000) at 4 degree overnight. After washing, membrane was incubated in anti-mouse-HRP in blocking buffer (1:10000) for 1h at room temperature. Membrane was developed in ECL and imaged on a Biorad chemiluminescence detection system.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: 293T

GAPDH Antibody Western Blot validation (1:5000 dilution) in 293T (Cat no:60004-1-Ig)

FH

Eiko (Verified Customer) (10-16-2020)

This antibody worked quite well in my western blotting. I used 5% skim milk TBS-T for blocking and antibody dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: hTERT-RPE1

GAPDH Antibody Western Blot validation (1:2000 dilution) in hTERT-RPE1 (Cat no:60004-1-Ig)

FH

Tom (Verified Customer) (09-28-2020)

HEK293T lysate (10ug) ran on 4-20% gradient gel. Blocking in 5% BSA for 1 hour, followed by HSP27 (1:5000 in block) overnight at 4 degrees. Goat anti-mouse HRP (1:10,000) for 1 hour in block.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293T

GAPDH Antibody Western Blot validation (1:5000 dilution) in HEK293T (Cat no:60004-1-Ig)

FH

Federica (Verified Customer) (06-08-2020)

Excellent antibody.I used in WB on HeLa lysate and the results was extremely clear. No unspecific bands detected. Perfect as loading control.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: HeLa cells

GAPDH Antibody Western Blot, validation (1:10000 dilution) in HeLa cells (Cat no:60004-1-Ig)

FH

Alinda (Verified Customer) (01-27-2020)

Good antibody for Western BLOT. antibody diluted in 5%milk.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK 293T

GAPDH Antibody Western Blot, validation (1:1000 dilution) in HEK 293T (Cat no:60004-1-Ig)

FH

Aamir (Verified Customer) (01-19-2020)

Good signal for WB

• Applications: Western Blot,
• Cell Tissue Type: HEKs

FH

David (Verified Customer) (01-13-2020)

Excellent antibody. Single band at the correct molecular weight. No background and can be used at high dilutions.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: SH-SY5Y

FH

saswati (Verified Customer) (01-08-2020)

Need a 1 hr block with 5% milk / TBST as usual.1 hr room temp with the same blocking media with a 1:8000 dilution, followed by secondary anti mouse ( same dilution) , HRP conjugated 1 hr room temp.Detect and document.4 is rating , since a 1:8000 fold is tad bit low, have used better dilution ( 1:100,000) in the past, however that product is not available these days

• Applications: Western Blot,
• Primary Antibody Dilution: 1 to 8000
• Cell Tissue Type: MDA-MB-231

FH

Emma (Verified Customer) (12-22-2019)

This antibody works well detecting GAPDH in 3t3-L1 and primary mouse embryonic fibroblasts.

• Applications: Western Blot,
• Cell Tissue Type: 3t3-L1, pMEF

FH

Nhu Quynh (Verified Customer) (12-16-2019)

High afffinity and specific antibody. Useful for protein controls in western blots.

• Applications: Western Blot,
• Cell Tissue Type: Cardiomyocytes

FH

Jia (Verified Customer) (11-12-2019)

This antibody works really well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: CHO-K1 cell

GAPDH Antibody Western Blot, validation (1:1000 dilution) in CHO-K1 cell (Cat no:60004-1-Ig)

FH

Juan (Verified Customer) (10-20-2019)

WB, it works well in 1:10000 dilution.

• Applications: Western Blot,

FH

Iram (Verified Customer) (10-01-2019)

Working excellent

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Pancreatic and Colon cancer cells

GAPDH Antibody Western Blot, validation (1:5000 dilution) in Pancreatic and Colon cancer cells (Cat no:60004-1-Ig)

FH

RICHA (Verified Customer) (09-25-2019)

Excellent antibody. Clean and specific

• Applications: Western Blot,
• Primary Antibody Dilution: 1:25000
• Cell Tissue Type: Protein from mouse tail tendon

GAPDH Antibody Western Blot, validation (1:25000 dilution) in Protein from mouse tail tendon (Cat no:60004-1-Ig)

FH

Pau (Verified Customer) (09-02-2019)

Excellent and clean Western blots.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293T

FH

Tanusree (Verified Customer) (08-14-2019)

The antibody works good in mouse brain tissue in western blotting.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

FH

Praveen (Verified Customer) (04-15-2019)

Good

• Applications: Western Blot,
• Cell Tissue Type: Ac16

GAPDH Antibody Western Blot, validation ( dilution) in Ac16 (Cat no:60004-1-Ig)

FH

Shubham (Verified Customer) (04-15-2019)

Very good

• Applications: Western Blot,
• Cell Tissue Type: AC16

GAPDH Antibody Western Blot, validation ( dilution) in AC16 (Cat no:60004-1-Ig)

FH

Vanessa (Verified Customer) (04-14-2019)

Total cell lysate (5-20 ug) was resolved on 4-12% Bis-Tris gels and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% BSA in PBS/0.1% Tween-20) for 1h. Membrane was incubated with anti-GAPDH in blocking buffer (1:5000) at 4C overnight. After washing, membrane was incubated in anti-mouse-HRP in blocking bufffer (1:10000) for 2h at room temperature. Membrane was developed in ECF reagent and imaged on a chemiluminescence detection system.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: brain

FH

Damaris (Verified Customer) (04-03-2019)

Excellent antibody! It gives clean and specific results

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: brain lysate

GAPDH Antibody Western Blot, validation (1:1000 dilution) in brain lysate (Cat no:60004-1-Ig)

FH

JIMMY (Verified Customer) (03-26-2019)

This antibody offers a good detection of GAPDH on mouse cells such as macrophages and microglia through western blots.

• Applications: Western Blot,

FH

Elisabeth (Verified Customer) (02-05-2019)

Total cell lysate (15 ug) was resolved on 4-12% Bis-Tris gels and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk in PBS/0.1% Tween-20) for 1h. Membrane was incubated with anti-GAPDH in blocking buffer (1:10,000) at 4C overnight. After washing, membrane was incubated in anti-mouse-HRP in blocking bufffer (1:5000) for 2h at room temperature. Membrane was developed in ECL reagent and imaged on a chemiluminescence detection system.

• Applications: Western Blot,
• Primary Antibody Dilution: 10,000
• Cell Tissue Type: HeLa cells lysate

GAPDH Antibody Western Blot, validation (10,000 dilution) in HeLa cells lysate (Cat no:60004-1-Ig)

FH

Stephen (Verified Customer) (01-31-2019)

great antibody for WB as a loading control used 1:20000

• Applications: Western Blot,
• Primary Antibody Dilution: 1:20000
• Cell Tissue Type: HeLa

GAPDH Antibody Western Blot, validation (1:20000 dilution) in HeLa (Cat no:60004-1-Ig)

FH

Kishor (Verified Customer) (01-30-2019)

I working good for Western Blotting (1:5000)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: H69, HUCCT-1

GAPDH Antibody Western Blot, validation (1:5000 dilution) in H69, HUCCT-1 (Cat no:60004-1-Ig)

FH

Wei (Verified Customer) (12-04-2018)

In muscle cells, GAPDH antibody shows two bands while in cancer cells there is only one band

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12

FH

Robert (Verified Customer) (12-03-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: neuronal cell culture

FH

Guillaume (Verified Customer) (11-21-2018)

This antibody is useful as a cytoplasmic control for cyto-nuclear extractions.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Prostate cancer cells

GAPDH Antibody Western Blot, validation (1/1000 dilution) in Prostate cancer cells (Cat no:60004-1-Ig)

FH

Ruth (Verified Customer) (11-21-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:40000
• Cell Tissue Type: Human cell line lysates

FH

Tingting (Verified Customer) (11-20-2018)

The bands of the GADPH looked very good! The antibody used in trophoblast cell and diluted in 1:1000.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Trophoblast

FH

marian (Verified Customer) (11-20-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: colon

GAPDH Antibody Western Blot, validation (1:1000 dilution) in colon (Cat no:60004-1-Ig)

FH

vanessa (Verified Customer) (11-20-2018)

very efficient product and great customer service

• Applications: Cell culture,

FH

Vermont (Verified Customer) (11-20-2018)

great sensitivity and reproducibility

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: macrophage

GAPDH Antibody Western Blot, validation (1:5000 dilution) in macrophage (Cat no:60004-1-Ig)

FH

Saswati (Verified Customer) (11-20-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:8000
• Cell Tissue Type: Human breast cancer

FH

Qunfeng (Verified Customer) (01-11-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Huh7 cells

FH

Sinu (Verified Customer) (01-10-2018)

Worked well in the western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human THP1

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