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GAPDH Antibody 1169 Publications

Mouse Monoclonal| Catalog number: 60004-1-Ig |CloneNo.: 1E6D9

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

GAPDH Antibody WB HepG2 cells 60004-1-Ig

Western blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody 60004-1-Ig at a dilution of 1:10000.

GAPDH Antibody WB HeLa cells 60004-1-Ig

Western blot of Hela cell with anti-GAPDH (60004-1-Ig) at various dilutions.

GAPDH Antibody IP HeLa Cells 60004-1-Ig

IP result of anti-GAPDH (60004-1-Ig for IP and Detection) with HeLa cell lysate.

GAPDH Antibody WB arabidopsis whole plant tissue 60004-1-Ig

arabidopsis, rice, wheat, corn whole plant tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig( GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours

GAPDH Antibody IHC human kidney tissue 60004-1-Ig

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 60004-1-Ig( GAPDH Antibody) at dilution of 1:50 (under 10x lens)

GAPDH Antibody IHC human kidney tissue 60004-1-Ig

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 60004-1-Ig( GAPDH Antibody) at dilution of 1:50 (under 40x lens)

GAPDH Antibody IF Ethacrynic acid treated HeLa cells 60004-1-Ig

Immunofluorescent analysis of Ethacrynic acid treated HeLa cells using 60004-1-Ig(GAPDH antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IgG (red).

GAPDH Antibody FC HeLa cells 60004-1-Ig

1X10^6 HeLa cells were stained with 0.2ug GAPDH antibody (60004-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IgG with dilution 1:100.

Species specificity:
human, mouse, rat, zebrafish, yeast, plant, beagle, chicken, cow, Cyprinus carpio, deer, Eelworm, Hamster, monkey
Positive WB detected in:
HeLa cells, HepG2 cells, zebrafish tissue, Raji cells, HEK-293 cells, whole Nematode tissue, soybean whole plant tissue, arabidopsis whole plant tissue, whole yeast cells
Positive IP detected in:
HeLa Cells
Positive IHC detected in:
human kidney tissue, human breast cancer tissue
Positive IF detected in:
Ethacrynic acid treated HeLa cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:2000-1:12000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
GAPDH fusion protein Ag0766
Full name:
glyceraldehyde-3-phosphate dehydrogenase
Calculated molecular weight:
36 kDa
Observed molecular weight:
36 kDa
GenBank accession number:
BC004109
Gene ID (NCBI):
2597
Gene symbol
GAPDH
Synonyms
G3PD, GAPD, GAPDH, OK/SW cl.12

Background

What is the molecular weight of GAPDH?

The molecular weight of GAPDH is 36 kD.


What is GAPDH?

GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase and is often referred to as a “housekeeping” 

protein. It is recognized as an important enzyme involved in metabolic pathways, aiding in the glycolytic 

production of ATP and pyruvate (PMID: 21895736).


What is the function of GAPDH?

GAPDH is responsible for catalyzing the phosphorylation of glyceraldehyde-3-phosphate into D-glycerate-1,3-biphosphate. While this function of GAPDH is considered its primary function, GAPDH is also involved, even 

critical, in other cell functions, some of which are still being studied. Recent studies have suggested that GAPDH 

is a key factor in regulating apoptosis, translocating to the nucleus under certain stressful conditions and acting 

as a signaling factor in oxidative stress-induced apoptosis. Other studies indicate that GAPDH plays a role in 

transporting vesicles from the endoplasmic reticulum to the Golgi body (PMIDs: 21895736, 12829261, 25859407, 

11035021).


Where in the cell is GAPDH localized?

GAPDH is expressed primarily in the cytosolic and membrane regions, with minimal expression in the nucleus 

(PMIDs: 21895736, 12829261).


Is there a reason to use an antibody targeting GAPDH if my research is not concerned with glycolysis or nuclear 

functions?

Yes! Because GAPDH is often highly expressed throughout different tissues and cell types, GAPDH expression is 

useful as a control for Western blots and RT-PCR.


What are the known protein-protein interactions of GAPDH?

GAPDH interacts with other proteins to regulate nuclear translocation and GAPDH catalytic activity. Some of 

these proteins include TPPP, SIAH1, CHP1, RILPL1/GOSPEL and FKBP6 (PMIDs: 21895736, 25859407).


Customer Reviews

Share your scientific insight on this antibody

GAPDH Mouse Monoclonal antibody

Qunfeng Wu, 01-11-2018
Applications
Western Blot,
Lot Number
10004129
Primary Antibody Dilution
1:10000
Secondary Antibody Dilution
1:10000
Cell/Tissue Type
Huh7 cells

GAPDH Mouse Monoclonal antibody

Sinu John, 01-10-2018
Applications
Western Blot,
Lot Number
1
Primary Antibody Dilution
1:1000
Secondary Antibody Dilution
1:5000
Cell/Tissue Type
Human THP1

Worked well in the western blot

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