CoraLite®594-conjugated GAPDH Monoclonal antibody

GAPDH Monoclonal Antibody for WB, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, zebrafish, yeast, plant

Applications

WB, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E6D9

Cat no : CL594-60004

Synonyms

G3PD, GAPD, GAPDH, OK/SW cl.12



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

CL594-60004 targets GAPDH in WB, FC (Intra) applications and shows reactivity with human, mouse, rat, zebrafish, yeast, plant samples.

Tested Reactivity human, mouse, rat, zebrafish, yeast, plant
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GAPDH fusion protein Ag0766
Full Name glyceraldehyde-3-phosphate dehydrogenase
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC004109
Gene Symbol GAPDH
Gene ID (NCBI) 2597
RRIDAB_2919886
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Protocols

Product Specific Protocols
WB protocol for CL594 GAPDH antibody CL594-60004Download protocol
FC protocol for CL594 GAPDH antibody CL594-60004Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

BMC Cancer

Oridonin-induced ferroptosis and apoptosis: a dual approach to suppress the growth of osteosarcoma cells

Authors - Feifan Zhang
mouseWB

Brain Sci

Itaconate Attenuates Neuroinflammation and Exerts Dopamine Neuroprotection in Parkinson's Disease through Inhibiting NLRP3 Inflammasome

Authors - Guoqing Sun
humanWB

Cell Chem Biol

Identification of KLHDC2 as an efficient proximity-induced degrader of K-RAS, STK33, β-catenin, and FoxP3

Authors - Sascha Röth
mouseWB

Int J Mol Med

Flavin containing monooxygenase 2 regulates renal tubular cell fibrosis and paracrine secretion via SMURF2 in AKI‑CKD transformation

Authors - Longfei Wang