CoraLite® Plus 488-conjugated GFAP Monoclonal antibody

GFAP Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4B2E10

Cat no : CL488-60190

Synonyms

GFAP



Tested Applications

Positive IF detected inmouse brain tissue
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

This antibody is not recommended for immunocytofluorescent assays. It is not suitable for frozen sections.

Published Applications

IFSee 4 publications below

Product Information

CL488-60190 targets GFAP in IF, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivitymouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GFAP fusion protein Ag10452
Full Name glial fibrillary acidic protein
Calculated Molecular Weight 432 aa, 50 kDa
GenBank Accession NumberBC013596
Gene Symbol GFAP
Gene ID (NCBI) 2670
RRIDAB_2883116
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central nervous system (CNS). GFAP is one of the main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis. GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963). Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916), which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes. Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963). GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 GFAP antibody CL488-60190Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Neurosci Res

Neuronal deficiency of hypoxia-inducible factor 2α increases hypoxic-ischemic brain injury in neonatal mice.

Authors - Dawei Sun
mouseIF

Front Pharmacol

Extracellular Vesicle-Mediated Delivery of Ultrasmall Superparamagnetic Iron Oxide Nanoparticles to Mice Brain.

Authors - Naseer A Kutchy
ratIF

Brain Res

Effects of cannabinoid (CBD) on blood brain barrier permeability after brain injury in rats.

Authors - Hongyan Jiang
mouseIF

Neural Regen Res

Transient neurogenesis in ischemic cortex from Sox2+ astrocytes

Authors - Jia-Lei Yang