CoraLite®488-conjugated GFAP Monoclonal antibody

GFAP Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

Publications

3

CloneNo.

4B2E10

Cat no : CL488-60190

Synonyms

GFAP

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Validation Data Gallery

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  IF Staining of mouse brain using CL488-60190

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-60190 (GFAP antibody) at dilution of 1:100.

• 

  IF Staining of mouse brain using CL488-60190

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-60190 (GFAP antibody) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:200

Sample-dependent, check data in validation data gallery

This antibody is not recommended for immunocytofluorescent assays. It is not suitable for frozen sections.

Published Applications

• IF: See 3 publications below

Product Information

CL488-60190 targets GFAP in IF applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: GFAP fusion protein Ag10452
• Full Name: glial fibrillary acidic protein
• Calculated molecular weight: 432 aa, 50 kDa
• GenBank accession number: BC013596
• Gene symbol: GFAP
• Gene ID (NCBI): 2670
• Conjugate: CoraLite®488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 488 nm / 515 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central nervous system (CNS). GFAP is one of the main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis. GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963). Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916), which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes. Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963). GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Publications

Species	Application	Title
rat	IF	Brain Res; Effects of cannabinoid (CBD) on blood brain barrier permeability after brain injury in rats.; Authors - Hongyan Jiang
mouse	IF	Front Pharmacol; Extracellular Vesicle-Mediated Delivery of Ultrasmall Superparamagnetic Iron Oxide Nanoparticles to Mice Brain.; Authors - Naseer A Kutchy
mouse	IF	J Neurosci Res; Neuronal deficiency of hypoxia-inducible factor 2α increases hypoxic-ischemic brain injury in neonatal mice.; Authors - Dawei Sun