GGA2 antibody (10356-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 10356-1-AP (same clone as 10356-1-PBS)
WB result of GGA2 antibody (10356-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GGA2 transfected HeLa cells. This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 10356-1-AP (same clone as 10356-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10356-1-AP (GGA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.
WB analysis of human kidney using 10356-1-AP (same clone as 10356-1-PBS)
human kidney tissue were subjected to SDS PAGE followed by western blot with 10356-1-AP (GGA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.
WB analysis of mouse adipose using 10356-1-AP (same clone as 10356-1-PBS)
mouse adipose tissue were subjected to SDS PAGE followed by western blot with 10356-1-AP (GGA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 10356-1-AP (same clone as 10356-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10356-1-AP (GGA2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 10356-1-AP (same clone as 10356-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10356-1-AP (GGA2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 10356-1-AP (same clone as 10356-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GGA2 antibody (10356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.

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Tested Applications
Product Information

Product Information

10356-1-PBS targets GGA2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GGA2 fusion protein Ag0370 Predict reactive species
Full Name	golgi associated, gamma adaptin ear containing, ARF binding protein 2
Calculated Molecular Weight	67 kDa
Observed Molecular Weight	67 kDa
GenBank Accession Number	BC000284
Gene Symbol	GGA2
Gene ID (NCBI)	23062
RRID	AB_2110735
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UJY4
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

GGA2, also known as VEAR, belongs to the GGA protein family and regulates the trafficking of proteins between the trans-Golgi network and the lysosome. GGA2 has an amino-terminal VHS domain in its N terminus which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins.