GGA2 Polyclonal antibody

GGA2 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10356-1-AP

Synonyms

GGA2, KIAA1080, VEAR

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Validation Data Gallery

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  WB analysis of mouse brain using 10356-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10356-1-AP (GGA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 10356-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 10356-1-AP (GGA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse adipose using 10356-1-AP

  mouse adipose tissue were subjected to SDS PAGE followed by western blot with 10356-1-AP (GGA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 10356-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GGA2 antibody (10356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human kidney tissue, mouse adipose tissue
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below

Product Information

10356-1-AP targets GGA2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GGA2 fusion protein Ag0370
• Full Name: golgi associated, gamma adaptin ear containing, ARF binding protein 2
• Calculated molecular weight: 67 kDa
• Observed molecular weight: 67 kDa
• GenBank accession number: BC000284
• Gene symbol: GGA2
• Gene ID (NCBI): 23062
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	WB	J Cell Biol; Cargo-selective SNX-BAR proteins mediate retromer trimer independent retrograde transport.; Authors - Arunas Kvainickas
rat	WB	Front Cardiovasc Med; Modular Screening Reveals Driver Induced Additive Mechanisms of Baicalin and Jasminoidin on Cerebral Ischemia Therapy.; Authors - Bing Li