GLUD1 Monoclonal antibody

GLUD1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

4G10D3

Cat no : 67026-1-Ig

Synonyms

GDH, GDH 1, GDH1, GLUD, GLUD1, glutamate dehydrogenase 1



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, L02 cells, HuH-7 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67026-1-Ig targets GLUD1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GLUD1 fusion protein Ag6179
Full Name glutamate dehydrogenase 1
Calculated molecular weight 61 kDa
Observed molecular weight 45-55 kDa
GenBank accession numberBC040132
Gene symbol GLUD1
Gene ID (NCBI) 2746
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Human glutamate dehydrogenase (GDH), an enzyme central to the metabolism of glutamate, is known to exist in housekeeping and nerve tissue-specific isoforms encoded by the GLUD1 and GLUD2 genes, respectively. It catalyses the reversible inter-conversion of glutamate to alpha-ketoglutarate and ammonia, thus interconnecting amino acid and carbohydrate metabolism. GLUD1 might contribute to the formation of specific synapses in the hippocampus such as those formed by the projecting neurons of the entorhinal cortex(PMID: 22138648). GLUD1 has a calculated molecular mass of 61 kDa and an apparent molecular mass of 45-55 kDa with the 53aa transit peptide removed.

Protocols

Product Specific Protocols
WB protocol for GLUD1 antibody 67026-1-IgDownload protocol
IHC protocol for GLUD1 antibody 67026-1-IgDownload protocol
IF protocol for GLUD1 antibody 67026-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols