CoraLite®594-conjugated GLUD1 Monoclonal antibody

GLUD1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4G10D3

Cat no : CL594-67026

Synonyms

GDH, GDH 1, GDH1, GLUD, GLUD1, glutamate dehydrogenase 1

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Validation Data Gallery

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  IF Staining of human liver cancer using CL594-67026

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594-conjugated GLUD1 antibody (CL594-67026, Clone: 4G10D3 ) at dilution of 1:200.

• 

  IF Staining of human liver cancer using CL594-67026

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594-conjugated GLUD1 antibody (CL594-67026, Clone: 4G10D3 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human liver cancer tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67026 targets GLUD1 in IF applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GLUD1 fusion protein Ag6179
• Full Name: glutamate dehydrogenase 1
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BC040132
• Gene Symbol: GLUD1
• Gene ID (NCBI): 2746
• RRID: AB_2920074
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human glutamate dehydrogenase (GDH), an enzyme central to the metabolism of glutamate, is known to exist in housekeeping and nerve tissue-specific isoforms encoded by the GLUD1 and GLUD2 genes, respectively. It catalyses the reversible inter-conversion of glutamate to alpha-ketoglutarate and ammonia, thus interconnecting amino acid and carbohydrate metabolism. GLUD1 might contribute to the formation of specific synapses in the hippocampus such as those formed by the projecting neurons of the entorhinal cortex(PMID: 22138648). GLUD1 has a calculated molecular mass of 61 kDa and an apparent molecular mass of 45-55 kDa with the 53aa transit peptide removed.