GM-CSF Recombinant monoclonal antibody

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  WB analysis of HEK-293T using 86923-1-RR

  Non-transfected and rat GM-CS Transfected HEK-293T cells were subjected to SDS PAGE followed by western blot with 86923-1-RR (GM-CSF/CSF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86923-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86923-1-RR against Rat GM-CSF were performed. The affinity constant is 35.8 pM.

GM-CSF Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86923-1-RR

Clone No.243117B1

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, ELISA

Synonyms

Colony-stimulating factor, CSF, Csf2, Csfgm, Granulocyte-macrophage colony-stimulating factor

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Tested Applications

• Positive WB detected in: Transfected with Rat GM-CSF/CSF2 HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86923-1-RR targets GM-CSF in WB, ELISA applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3202

  Product name: Recombinant Rat GM-CSF protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 18-144 aa of NM_053852.1

  Sequence: APTRSPNPVTRPWKHVDAIKEALSLLNDMRALENEKNEDVDIISNEFSIQRPTCVQTRLKLYKQGLRGNLTKLNGALTMIASHYQTNCPPTPETDCEIEVTTFEDFIKNLKGFLFDIPFDCWKPVQK

• Full Name: colony stimulating factor 2 (granulocyte-macrophage)
• Calculated Molecular Weight: 17 kDa
• GenBank Accession Number: NM_053852.1
• Gene Symbol: Csf2
• Gene ID (NCBI): 116630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P48750
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gm-csf, also known as Csf2, is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. Gm-csf was first characterized as a hematopoietic growth factor that stimulates the proliferation of myeloid cells from bone-marrow progenitors. Gm-csf is now recognized as an important activating and differentiation factor for immune cells, and is essential for a wide range of biological processes in both innate and adaptive immunity. Gm-csf has been shown to protect against pulmonary infection and intestinal inflammation, and it is necessary for normal pulmonary and colon homeostasis.