GP2 Polyclonal antibody

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  WB analysis using 13493-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13493-1-AP (GP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13493-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13493-1-AP (GP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse pancreas using 13493-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13493-1-AP (GP2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GP2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13493-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Pancreatic zymogen granule membrane protein GP-2, ZAP75

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat pancreas tissue, rat brain tissue, mouse pancreas tissue
• Positive IHC detected in: mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13493-1-AP targets GP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4317

  Product name: Recombinant human GP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-360 aa of BC032693

  Sequence: MERMVGSGLLWLALVSCILTQASAVQRDPSTVEDKCEKACRPEEECLALNSTWGCFCRQDLNSSDVHSLQPQLDCGPREIKVKVDKCLLGGLGLGEEVIAYLRDPNCSSILQTEERNWVSVTSPVQASACRNILERNQTHAIYKNTLSLVNDFIIRDTILNINFQCAYPLDMKVSLQAALQPIVSSLNVSVDGNGEFIVRMALFQDQNYTNPYQGDAVELSVESVLYVGAILEQGDTSRFNLVLRNCYATPTEDKADLVKYFIIRNSCSNQRDSTIHVEENGQSSESRFSVQMFMFAGHYDLVFLHCEIHLCDSLNEQCQPSCSRSQVRSEVPAIDLARVLDLGPITRRGAQSPGVMNGT

• Full Name: glycoprotein 2 (zymogen granule membrane)
• Calculated Molecular Weight: 530 aa, 58 kDa
• Observed Molecular Weight: 75-85 kDa
• GenBank Accession Number: BC032693
• Gene Symbol: GP2
• Gene ID (NCBI): 2813
• RRID: AB_3669164
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P55259
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pancreatic secretory granule membrane major glycoprotein (GP2) is the most abundant membrane component of zymogen granules secreted by pancreatic acinar cells (PMID: 8190715). GP2 associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. It is released from the membrane of mature zymogen granules by an enzymatic mechanism (PMID: 2011597). GP2 plays a role in defense against adhesive and invasive commensal bacteria during intestinal inflammation (PMID: 33594081). GP2 is preferentially expressed in acinar cell carcinomas of the pancreas but the glycoprotein can rarely also be expressed in a variety of other tumor entities (PMID: 36137400). The apparent molecular weight of GP2 is 70-100 kDa, which is larger than the calculated molecular weight, due to glycosylation (PMID: 19627615; 30186250).