GP2 Polyclonal antibody

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  WB analysis using 13493-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13493-1-AP (GP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

GP2 Polyclonal Antibody for WB, ELISA

Cat No. 13493-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ZAP75, Pancreatic zymogen granule membrane protein GP-2

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Tested Applications

• Positive WB detected in: rat pancreas tissue, mouse pancreas tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13493-1-AP targets GP2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GP2 fusion protein Ag4317
• Full Name: glycoprotein 2 (zymogen granule membrane)
• Calculated Molecular Weight: 530 aa, 58 kDa
• Observed Molecular Weight: 75-85 kDa
• GenBank Accession Number: BC032693
• Gene Symbol: GP2
• Gene ID (NCBI): 2813
• RRID: AB_3669164
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P55259
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pancreatic secretory granule membrane major glycoprotein (GP2) is the most abundant membrane component of zymogen granules secreted by pancreatic acinar cells (PMID: 8190715). GP2 associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. It is released from the membrane of mature zymogen granules by an enzymatic mechanism (PMID: 2011597). GP2 plays a role in defense against adhesive and invasive commensal bacteria during intestinal inflammation (PMID: 33594081). GP2 is preferentially expressed in acinar cell carcinomas of the pancreas but the glycoprotein can rarely also be expressed in a variety of other tumor entities (PMID: 36137400). The apparent molecular weight of GP2 is 70-100 kDa, which is larger than the calculated molecular weight, due to glycosylation (PMID: 19627615; 30186250).