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GPR132 Polyclonal antibody

GPR132 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 17026-1-AP

Synonyms

G protein coupled receptor 132, G2 accumulation protein, G2A, GPR132



Tested Applications

Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17026-1-AP targets GPR132 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR132 fusion protein Ag10325
Full Name G protein-coupled receptor 132
Calculated Molecular Weight 380 aa, 43 kDa
GenBank Accession NumberBC084546
Gene Symbol GPR132
Gene ID (NCBI) 29933
RRIDAB_2113375
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR132, also known as G2A (G2 accumulation protein), belongs to the G-protein coupled receptor (GPCR) 1 family. GPR132 is a DNA damage and stress inducible GPCR that blocks cells in G2/M. It functions at the G2/M checkpoint to delay mitosis and may serve as a mechanism for T and B cells and other cell types to slow their proliferation and repair damaged DNA to ensure proper replication (PMID: 9770487). Lysophosphatidylcholine (LPC) is a high affinity ligand for GPR132 (PMID: 15653487).

Protocols

Product Specific Protocols
IHC protocol for GPR132 antibody 17026-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Eur Acad Dermatol Venereol

Lysophosphatidylcholine facilitates the pathogenesis of psoriasis through activating keratinocytes and T cells differentiation via glycolysis

Authors - Panpan Liu
mouse

Front Oncol

Lactate increases tumor malignancy by promoting tumor small extracellular vesicles production via the GPR81-cAMP-PKA-HIF-1α axis

Authors - Man Luo
  • KD Validated
mouse

Neurosci Res

Proton-sensing receptor GPR132 facilitates migration of astrocytes.

Authors - Ken Matoba
human

J Hepatobiliary Pancreat Sci

Partial characterization of proapoptotic action of biliary deteriorated lipids on biliary epithelial cells in pancreaticobiliary diseases.

Authors - Fujita Keiko K
mouse

Nat Commun

Lysophosphatidylserine induces necrosis in pressure overloaded male mouse hearts via G protein coupled receptor 34

Authors - Ryuta Sugihara