GPR37/Pael-R Polyclonal antibody

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  WB analysis of mouse brain using 14820-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14820-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14820-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 14820-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14820-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 14820-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GPR37/Pael-R antibody (14820-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14820-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GPR37/Pael-R antibody (14820-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 14820-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GPR37/Pael-R antibody (14820-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

GPR37/Pael-R Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 14820-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

GPR37, Pael-R, EDNRBL, Endothelin B receptor-like protein 1, ETBR LP 1

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3200
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IF: See 4 publications below

Product Information

14820-1-AP targets GPR37/Pael-R in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPR37/Pael-R fusion protein Ag6591
• Full Name: G protein-coupled receptor 37 (endothelin receptor type B-like)
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC040007
• Gene Symbol: GPR37
• Gene ID (NCBI): 2861
• RRID: AB_2232549
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15354
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The orphan G protein-coupled receptor GPR37, also known as PAELR (parkin-associated endothelin receptor-like receptor) or ETBR-LP-1 (endothelin B receptor-like protein 1), is a 613 aa, multi-pass membrane protein predominantly expressed in the brain. It is a substrate of parkin (PARK2). When overexpressed in cells, GPR37 tends to become insoluble, unfolded, and ubiquitinated in vivo. Accumulation of the unfolded protein may lead to dopaminergic neuronal death in juvenile Parkinson disease (JPD). This antibody recognizes endogenous GPR37, which migrates as an approximately 50-55 kDa band in SDS-PAGE (PMID:17519329).

Publications

Species	Application	Title
human	WB	Mol Syst Biol; Systematic protein-protein interaction mapping for clinically relevant human GPCRs.; Authors - Kate Sokolina
mouse	WB	Proc Natl Acad Sci U S A; GPR37 and GPR37L1 are receptors for the neuroprotective and glioprotective factors prosaptide and prosaposin.; Authors - Meyer Rebecca C RC
human	WB	Oncogene; GPR37 promotes colorectal cancer liver metastases by enhancing the glycolysis and histone lactylation via Hippo pathway; Authors - Jiamin Zhou
mouse,human	WB,IF	EMBO Rep; Parkinson's disease-associated receptor GPR37 is an ER chaperone for LRP6.; Authors - Birgit S Berger ; KD Validated
	WB	Glia; Glio- and neuro-protection by prosaposin is mediated by orphan G-protein coupled receptors GPR37L1 and GPR37.; Authors - Beihui Liu
mouse	WB	Biomedicines; Osteocalcin Alleviates Lipopolysaccharide-Induced Acute Inflammation via Activation of GPR37 in Macrophages.; Authors - Zhengjiang Qian ; KO Validated