GPX1 Polyclonal antibody

GPX1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (4)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 29329-1-AP

Synonyms

glutathione peroxidase 1, GPx 1, GPX1, GSHPx 1, GSHPX1

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Validation Data Gallery

• 

  WB analysis using 29329-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29329-1-AP (GPX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29329-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29329-1-AP (GPX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29329-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29329-1-AP (GPX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, THP-1 cells, mouse kidney tissue, rat kidney tissue, SMMC-7721 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 1 publications below

Product Information

29329-1-AP targets GPX1 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPX1 fusion protein Ag31101
• Full Name: glutathione peroxidase 1
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC000742
• Gene Symbol: GPX1
• Gene ID (NCBI): 2876
• RRID: AB_2918283
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gpx protein, known as selenoprotein, consists selenium in its catalytic site. Gpx1 is an abundant isoform, which involves in scavenging endogenous ROS using electron provided by reduced glutathione and ubiquitously expressed the cytoplasm and mitochondria of all cell types. (PMID: 30632027)

Publications

Species	Application	Title
mouse	WB	Mol Ther; CircRNA Samd4 induces cardiac repair after myocardial infarction by blocking mitochondria-derived ROS output.; Authors - Hao Zheng
human,mouse	WB,IF	Cell Death Discov; Allicin promotes functional recovery in ischemic stroke via glutathione peroxidase-1 activation of Src-Akt-Erk; Authors - Fei Zhuang ; KD Validated
mouse	WB	Molecules; Polygonatum cyrtonema Hua Polysaccharides Protect BV2 Microglia Relief Oxidative Stress and Ferroptosis by Regulating NRF2/HO-1 Pathway; Authors - Jiayu Li
rat	WB	Pharmaceuticals (Basel); Adrenomedullin Improves Cardiac Remodeling and Function in Obese Rats with Hypertension.; Authors - Pei Qian
rat	WB	Acta Diabetol; Adrenomedullin ameliorates palmitic acid-induced insulin resistance through PI3K/Akt pathway in adipocytes.; Authors - Hang-Bing Dai
mouse	WB	bioRxiv; WDR23 mediates NRF2 proteostasis and cytoprotective capacity in the hippocampus; Authors - Jiahui Liu