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GR repeat Antibody 9 Publications

Rabbit Polyclonal| Catalog number: 23978-1-AP

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Con: 36 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, Dot blot, ELISA
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GR repeat Antibody WB ag16331 23978-1-AP

Recombinant protein were subjected to SDS PAGE followed by western blot with 23978-1-AP( GR repeat Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Species specificity:
human, Drosophila, zebrafish
Positive WB detected in:
Recombinant protein
Recommended dilution:
WB : 1:500-1:2000
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
GR repeat
Calculated molecular weight:
Observed molecular weight:
GenBank accession number:
Gene ID (NCBI):
Gene symbol
Synonyms
C9RAN, GGGCC, GR repeat, GRGRGRGRG, GRGRGRGRGR, Poly-GR

Background

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the GR repeated sequence. The recombinant proteins GST-SNRPD1 and 6*His tag- SNRPD1 which contains the sequence “GRGRGRGRGRGRGRGRGR” were used to detect this antibody. Both proteins can be recognized by the antibody 23978-1-AP.


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