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  • KD/KO Validated

GR repeat Polyclonal antibody

GR repeat Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, Dot Blot, ELISA

Conjugate

Unconjugated

Cat no : 23978-1-AP

Synonyms

C9RAN, GGGCC, GR repeat, GRGRGRGRG, GRGRGRGRGR, Poly-GR



Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

23978-1-AP targets GR repeat in WB, IHC, IF, Dot Blot, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, Drosophila, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name GR repeat
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the GR repeated sequence. The recombinant proteins GST-SNRPD1 and 6*His tag- SNRPD1 which contains the sequence "GRGRGRGRGRGRGRGRGR" were used to detect this antibody. Both proteins can be recognized by the antibody 23978-1-AP.

Protocols

Product Specific Protocols
WB protocol for GR repeat antibody 23978-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nature

The C9orf72 repeat expansion disrupts nucleocytoplasmic transport.

Authors - Ke Zhang
DrosophilaWB

Science

C9orf72 repeat expansions cause neurodegeneration in Drosophila through arginine-rich proteins.

Authors - Sarah Mizielinska
humanIF

Cell

Stress Granule Assembly Disrupts Nucleocytoplasmic Transport.

Authors - Ke Zhang
  • KD Validated
humanIHC

Nat Neurosci

RPS25 is required for efficient RAN translation of C9orf72 and other neurodegenerative disease-associated nucleotide repeats.

Authors - Shizuka B Yamada
zebrafishDot Blot

Acta Neuropathol

A zebrafish model for C9orf72 ALS reveals RNA toxicity as a pathogenic mechanism.

Authors - Bart Swinnen
humanIHC

Acta Neuropathol

Increased prevalence of granulovacuolar degeneration in C9orf72 mutation.

Authors - Yuichi Riku