GR repeat Polyclonal antibody

• 

  WB analysis of Recombinant protein using 23978-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 23978-1-AP (GR repeat Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

GR repeat Polyclonal Antibody for WB, ELISA

Cat No. 23978-1-AP

Publications(30)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, Dot Blot, ELISA

Synonyms

C9RAN, GGGCC, GR repeat, GRGRGRGRG, GRGRGRGRGR, Poly-GR

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 7 publications below
• IF: See 11 publications below

Product Information

23978-1-AP targets GR repeat in WB, IHC, IF, Dot Blot, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, zebrafish, drosophila
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: GR repeat
• RRID: AB_2879387
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the GR repeated sequence. The recombinant proteins GST-SNRPD1 and 6*His tag- SNRPD1 which contains the sequence "GRGRGRGRGRGRGRGRGR" were used to detect this antibody. Both proteins can be recognized by the antibody 23978-1-AP.

Publications

Species	Application	Title
	WB	Nature; The C9orf72 repeat expansion disrupts nucleocytoplasmic transport.; Authors - Ke Zhang
Drosophila	WB	Science; C9orf72 repeat expansions cause neurodegeneration in Drosophila through arginine-rich proteins.; Authors - Sarah Mizielinska
human	IF	Cell; Stress Granule Assembly Disrupts Nucleocytoplasmic Transport.; Authors - Ke Zhang ; KD Validated
human	IHC	Nat Neurosci; RPS25 is required for efficient RAN translation of C9orf72 and other neurodegenerative disease-associated nucleotide repeats.; Authors - Shizuka B Yamada
human		Nat Commun; Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis; Authors - Ji Geng
mouse	IF	Neuron; PTPσ-mediated PI3P regulation modulates neurodegeneration in C9ORF72-ALS/FTD; Authors - Zhe Zhang