GRLF1 Polyclonal antibody

GRLF1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 26789-1-AP

Synonyms

ARHGAP35, GRF 1, GRF1, GRLF1, KIAA1722, P190 A, P190A, p190RhoGAP, Rho GAP p190A



Tested Applications

Positive WB detected inA549 cells, HeLa cells
Positive IHC detected inhuman colon tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26789-1-AP is GRLF1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRLF1 fusion protein Ag25353
Full Name glucocorticoid receptor DNA binding factor 1
Observed molecular weight 172 kDa
GenBank accession numberBC150257
Gene symbol GRLF1
Gene ID (NCBI) 2909
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GRLF1 antibody 26789-1-APDownload protocol
IHC protocol for GRLF1 antibody 26789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

Feedback-Driven Mechanisms Between Phosphorylated Caveolin-1 and Contractile Actin Assemblies Instruct Persistent Cell Migration.

Authors - Xuemeng Shi
humanWB,IF

Oncogene

Ubiquitin ligase TRIM65 promotes colorectal cancer metastasis by targeting ARHGAP35 for protein degradation.

Authors - Daici Chen