GSDMD Polyclonal antibody

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  WB analysis using 31942-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31942-1-AP (Gsdmd antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

GSDMD Polyclonal Antibody for WB, ELISA

Cat No. 31942-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

AW558049, DF5L, Dfna5l, gasdermin D, Gsdmdc1

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Tested Applications

• Positive WB detected in: EL-4 cells, J774A.1 cells, NIH/3T3 cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31942-1-AP targets GSDMD in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSDMD fusion protein Ag36922
• Full Name: gasdermin D
• Calculated Molecular Weight: 53kDa，487aa
• Observed Molecular Weight: 50-53 kDa
• GenBank Accession Number: NM_026960.4
• Gene Symbol: Gsdmd
• Gene ID (NCBI): 69146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9D8T2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can only dectect full length GSDMD.