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GSTP1 Polyclonal antibody

GSTP1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 15902-1-AP

Synonyms

DFN7, FAEES3, Glutathione S transferase P, glutathione S transferase pi 1, GST class pi, GST3, GSTP1, GSTP1 1, PI



Tested Applications

Positive WB detected inCOLO 320 cells, mouse brain tissue, Jurkat cells, PC-3 cells, K-562 cells, human brain tissue, rat brain tissue, HeLa cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman skin tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15902-1-AP targets GSTP1 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSTP1 fusion protein Ag8731
Full Name glutathione S-transferase pi 1
Calculated molecular weight 210 aa, 23 kDa
Observed molecular weight 23-28 kDa
GenBank accession numberBC010915
Gene symbol GSTP1
Gene ID (NCBI) 2950
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GSTP1 antibody 15902-1-APDownload protocol
IHC protocol for GSTP1 antibody 15902-1-APDownload protocol
IF protocol for GSTP1 antibody 15902-1-APDownload protocol
IP protocol for GSTP1 antibody 15902-1-APDownload protocol
FC protocol for GSTP1 antibody 15902-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Toxicol Pathol

Thioacetamide-induced Hepatocellular Necrosis Is Attenuated in Diet-induced Obese Mice.

Authors - Shirai Makoto M
mouseWB

Biochem Biophys Res Commun

Proteomic profiling of brain cortex tissues in a Tau transgenic mouse model of Alzheimer's disease.

Authors - Seong-Hun Chang
humanWB

Drug Des Devel Ther

MicroRNA-133b targets glutathione S-transferase π expression to increase ovarian cancer cell sensitivity to chemotherapy drugs.

Authors - Shuo Chen
humanWB

Food Chem Toxicol

Protection of cyanidin-3-glucoside against oxidative stress induced by acrylamide in human MDA-MB-231 cells.

Authors - Song Jian J
humanIHC

J Cancer

In Vitro and in Vivo Efficacy of NBDHEX on Gefitinib-resistant Human Non-small Cell Lung Cancer.

Authors - Huanhuan Sha
mouseWB

PLoS One

Quantitative proteomic analysis of Niemann-Pick disease, type C1 cerebellum identifies protein biomarkers and provides pathological insight.

Authors - Cologna Stephanie M SM