GUF1 Recombinant monoclonal antibody

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  WB analysis using 86747-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86747-1-RR (GUF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 86747-1-RR

  IP result of anti-GUF1 (IP:86747-1-RR, 4ug; Detection:86747-1-RR 1:2000) with HepG2 cells lysate 2000 ug.

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  Affinity and Kinetic Characterization of 86747-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86747-1-RR against Human GUF1 were performed. The affinity constant is 9.10 pM.

GUF1 Uni-rAb^® Recombinant Antibody for WB, IP, ELISA

Cat No. 86747-1-RR

Clone No.251449D6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

EC:3.6.5.-, EF-4, Elongation factor 4 homolog, GTPase GUF1, Ribosomal back-translocase

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Tested Applications

• Positive WB detected in: HepG2 cells, LNCaP cells, mouse liver tissue, rat liver tissue, mouse brain tissue, mouse pancreas tissue
• Positive IP detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86747-1-RR targets GUF1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag11579

  Product name: Recombinant human GUF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 318-669 aa of BC036768

  Sequence: VGYLIAGMKDVTEAQIGDTLCLHKQPVEPLPGFKSAKPMVFAGMYPLDQSEYNNLKSAIEKLTLNDSSVTVHRDSSLALGAGWRLGFLGLLHMEVFNQRLEQEYNASVILTTPTVPYKAVLSSSKLIKEHREKEITIINPAQFPDKSKVTEYLEPVVLGTIITPDEYTGKIMMLCEARRAVQKNMIFIDQNRVMLKYLFPLNEIVVDFYDSLKSLSSGYASFDYEDAGYQTAELVKMDILLNGNTVEELVTVVHKDKAHSIGKAICERLKDSLPRQLFEIAIQAAIGSKIIARETVKAYRKNVLAKCYGGDITRKMKLLKRQAEGKKKLRKIGNVEVPKDAFIKVLKTQSSK

• Full Name: GUF1 GTPase homolog (S. cerevisiae)
• Calculated Molecular Weight: 669 aa, 74 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC036768
• Gene Symbol: GUF1
• Gene ID (NCBI): 60558
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N442
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GUF1 (GUF1 GTPase), also known as EF-4 (elongation factor 4 homolog), is a 669 amino acid protein belonging to the GTP-binding elongation factor family and the LepA subfamily. Localizing to the mitochondrion inner membrane, GUF1 promotes mitochondrial protein synthesis and binds to mitochondrial ribosomes in a GTP-dependent manner. GUF1 may act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes.