GYS2 Polyclonal antibody

GYS2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22371-1-AP

Synonyms

glycogen synthase 2 (liver), GYS2



Tested Applications

Positive WB detected inmouse liver tissue, mouse kidney tissue, rat liver tissue
Positive IP detected inrat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22371-1-AP is GYS2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GYS2 fusion protein Ag18012
Full Name glycogen synthase 2 (liver)
Calculated Molecular Weight 703 aa, 81 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberBC126310
Gene Symbol GYS2
Gene ID (NCBI) 2998
RRIDAB_2879091
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GYS2 antibody 22371-1-APDownload protocol
IHC protocol for GYS2 antibody 22371-1-APDownload protocol
IP protocol for GYS2 antibody 22371-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

Glycogen metabolism links glucose homeostasis to thermogenesis in adipocytes.

Authors - Omer Keinan
ratWB

Carbohydr Polym

Liver fibrosis alters the molecular structures of hepatic glycogen

Authors - Yujun Wan
mouseWB

Mol Metab

Disrupted Liver Oxidative Metabolism in Glycine N-Methyltransferase-Deficient Mice is Mitigated by Dietary Methionine Restriction.

Authors - Ferrol I Rome
ratWB

Food Funct

l-Theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathways

Authors - Ling Lin
ratWB

Metabolism

Recurrent hypoglycemia increases hepatic gluconeogenesis without affecting glycogen metabolism or systemic lipolysis in rat

Authors - Zejian Liu
ratWB

Nutrients

Role of Epigallocatechin Gallate in Glucose, Lipid, and Protein Metabolism and L-Theanine in the Metabolism-Regulatory Effects of Epigallocatechin Gallate.

Authors - Ling Lin