Gephyrin/GPHN Recombinant monoclonal antibody

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• Gephyrin/GPHN Recombinant monoclonal antibody (87903-2-RR)

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  WB analysis using 87903-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87903-2-RR (GPHN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity of 87903-2-RR

  Biolayer interferometry (BLI) kinetic assays of 87903-2-RR against Human Gephyrin/GPHN were performed. The affinity constant is 2.54 nM.

Gephyrin/GPHN Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87903-2-RR

Clone No.260132F4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Gephyrin, GPHN, Domain E, Domain G, GEPH

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, A549 cells, LNCaP cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87903-2-RR targets Gephyrin/GPHN in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3370

  Product name: Recombinant human GPHN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-315 aa of BC030016

  Sequence: MATEGMILTNHDHQIRVGVLTVSDSCFRNLAEDRSGINLKDLVQDPSLLGGTISAYKIVPDEIEEIKETLIDWCDEKELNLILTTGGTGFAPRDVTPEATKEVIEREAPGMALAMLMGSLNVTPLGMLSRPVCGIRGKTLIINLPGSKKGSQECFQFILPALPHAIDLLRDAIVKVKEVHDELEDLPSPPPPLSPPPTTSPHKQTEDKGVQCEEEEEEKKDSGVASTEDSSSSHITAAAIAAKKHPFYTSPAVVMAHGEQPIPGLINYSHHSTDERIPDSIISRGVQVLPRDTASLSTTPSESPRAQATSRLSTA

• Full Name: gephyrin
• Calculated Molecular Weight: 769 aa, 83 kDa
• Observed Molecular Weight: 93 kDa
• GenBank Accession Number: BC030016
• Gene Symbol: GPHN
• Gene ID (NCBI): 10243
• RRID: AB_3745818
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NQX3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gephyrin (GPHN) is an organizational protein that clusters and localizes the inhibitory glycine receptor (GlyR) and GABAA receptors to the microtubular matrix of the neuronal postsynaptic membrane. Mice deficient in gephyrin develop a hereditary molybdenum cofactor deficiency and a neurological phenotype that mimics startle disease (hyperekplexia). In non-neuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Two isoforms produced by alternative splicing have been described. The observed MW of Gephyrin is 93 kDa, larger than the predicated of 83 kDa, which may be due to the modifications on various phosphorylation sites.