HADHA Monoclonal antibody

• Featured Product
• KD/KO Validated

HADHA Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

3E9B1

Cat no : 60250-1-Ig

Synonyms

78 kDa gastrin binding protein, ECHA, HADH, HADHA, LCHAD, MTPA, TP ALPHA

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Validation Data Gallery

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  WB analysis of HEK293 cells using 60250-1-Ig

  WB result of HADHA specific antibody (60250-1-Ig, 1:4000) with si-control and si-HADHA transfected HEK293 cells.

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  WB analysis of A375 using 60250-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 60250-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 60250-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 60250-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60250-1-Ig(HADHA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 60250-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60250-1-Ig(HADHA antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 60250-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 60250-1-Ig(HADHA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 60250-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 60250-1-Ig(HADHA antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 60250-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60250-1-Ig (HADHA antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 60250-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 60250-1-Ig(HADHA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A375 cells, HEK293 cells
• Positive IHC detected in: human heart tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below

Product Information

The immunogen of 60250-1-Ig is HADHA Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HADHA fusion protein Ag1211
• Full Name: hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
• Calculated molecular weight: 83 kDa
• Observed molecular weight: 79 kDa
• GenBank accession number: BC009235
• Gene symbol: HADHA
• Gene ID (NCBI): 3030
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HADHA(Trifunctional enzyme subunit alpha, mitochondrial) is also named as HADH,78 kDa gastrin-binding protein.It belongs to the enoyl-CoA hydratase/isomerase family in the N-terminal section and the 3-hydroxyacyl-CoA dehydrogenase family in the central section.It harbors the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) and long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) and maternal acute fatty liver of pregnancy (AFLP).

Publications

Species	Application	Title
human	WB	Front Oncol; Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.; Authors - Jinghui Lu
mouse	WB	Proteomics; Quantitative Proteomic Study of Myocardial Mitochondria in Urea Transporter B Knockout Mice.; Authors - Yanwei Du ; KO Validated
human	WB	Cell Biosci; Exosomes from hyperglycemia-stimulated vascular endothelial cells contain versican that regulate calcification/senescence in vascular smooth muscle cells.; Authors - Shuang Li
mouse	WB	Science; Spermidine activates mitochondrial trifunctional protein and improves antitumor immunity in mice; Authors - Muna Al-Habsi
mouse	WB	RSC Adv; Glycolytic and lipid oxidative metabolic programs are essential for freshly-isolated regulatory T cells in mice with sepsis; Authors - Xiaomei Zhu