HIBCH Monoclonal antibody, PBS Only

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  WB analysis of HepG2 using 68157-1-Ig (same clone as 68157-1-PBS)

  WB result of HIBCH antibody (68157-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIBCH transfected HepG2 cells. This data was developed using the same antibody clone with 68157-1-PBS in a different storage buffer formulation.

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  WB analysis using 68157-1-Ig (same clone as 68157-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68157-1-Ig (HIBCH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68157-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68157-1-Ig (same clone as 68157-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIBCH antibody (68157-1-Ig, Clone: 1B7B8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68157-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

HIBCH Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 68157-1-PBS

Clone No.1B7B8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

HIBYL COA H, HIB-CoA hydrolase, HIB CoA hydrolase, EC:3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase

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Product Information

68157-1-PBS targets HIBCH in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: HIBCH fusion protein Ag6473
• Full Name: 3-hydroxyisobutyryl-Coenzyme A hydrolase
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC067822
• Gene Symbol: HIBCH
• Gene ID (NCBI): 26275
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6NVY1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HIBCH belongs to the enoyl-CoA hydratase/isomerase family. HIBCH has two isoforms with MW 43 kDa and 38 kDa. It is highly expressed in the liver and kidney, also detected in the heart, muscle and brain (at protein level), but not detected in the lung. Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (PMID: 8824301).