CoraLite® Plus 488-conjugated HIBCH Monoclonal antibody

HIBCH Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, rabbit, pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B7B8

Cat no : CL488-68157

Synonyms

HIB CoA hydrolase, HIBCH, HIBYL COA H

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-68157

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HIBCH antibody (CL488-68157, Clone: 1B7B8 ) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68157 targets HIBCH in IF applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

• Tested Reactivity: Human, mouse, rat, rabbit, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: HIBCH fusion protein Ag6473
• Full Name: 3-hydroxyisobutyryl-Coenzyme A hydrolase
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC067822
• Gene Symbol: HIBCH
• Gene ID (NCBI): 26275
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIBCH belongs to the enoyl-CoA hydratase/isomerase family. HIBCH has two isoforms with MW 43 kDa and 38 kDa. It is highly expressed in the liver and kidney, also detected in the heart, muscle and brain (at protein level), but not detected in the lung. Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (PMID: 8824301).