HIP1 Monoclonal antibody, PBS Only

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  WB analysis using 67163-1-Ig (same clone as 67163-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67163-1-Ig (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67163-1-Ig (same clone as 67163-1-PBS)

  WB result of HIP1 antibody (67163-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIP1 transfected HeLa cells. This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 67163-1-Ig (same clone as 67163-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67163-1-Ig (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 67163-1-Ig (same clone as 67163-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67163-1-Ig (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67163-1-Ig (same clone as 67163-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67163-1-Ig (same clone as 67163-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67163-1-Ig (same clone as 67163-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 67163-1-Ig (same clone as 67163-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using HIP1 antibody (67163-1-Ig, Clone: 3D3G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

HIP1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 67163-1-PBS

Clone No.3D3G3

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

HIP 1, HIP I, HIP1, ILWEQ

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Product Information

67163-1-PBS targets HIP1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse, pig samples.

• Tested Reactivity: Human, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17982

  Product name: Recombinant human HIP1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 435-764 aa of BC110545

  Sequence: AELDELRRQREDTEKAQRSLSEIERKAQANEQRYSKLKEKYSELVQNHADLLRKNAEVTKQVSMARQAQVDLEREKKELEDSLERISDQGQRKTQEQLEVLESLKQELATSQRELQVLQGSLETSAQSEANWAAEFAELEKERDSLVSGAAHREEELSALRKELQDTQLKLASTEESMCQLAKDQRKMLLVGSRKAAEQVIQDALNQLEEPPLISCAGSADHLLSTVTSISSCIEQLEKSWSQYLACPEDISGLLHSITLLAHLTSDAIAHGATTCLRAPPEPADSLTEACKQYGRETLAYLASLEEEGSLENADSTAMRNCLSKIKAIG

• Full Name: huntingtin interacting protein 1
• Calculated Molecular Weight: 1037 aa, 116 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC110545
• Gene Symbol: HIP1
• Gene ID (NCBI): 3092
• RRID: AB_2882459
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O00291
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HIP1 (huntingtin interacting protein-1) is a endocytic protein that plays a role in clathrin-mediated endocytosis and trafficking. HIP1 can interact with huntingtin (HTT) protein whose mutations cause Huntington's disease (HD), thus gets involved in neurodegeneration. By stabilizing receptor tyrosine kinases following ligand-induced endocytosis, HIP1 may act to promote cell survival. HIP1 is overexpressed in a variety of human cancers and may be a potential predictor for cancer progression and prognosis.