HIP1 Monoclonal antibody

• Featured Product
• KD/KO Validated

HIP1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3D3G3

Cat no : 67163-1-Ig

Synonyms

HIP 1, HIP I, HIP1, ILWEQ

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Validation Data Gallery

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  WB analysis using 67163-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67163-1-Ig (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67163-1-Ig

  WB result of HIP1 antibody (67163-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIP1 transfected HeLa cells.

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  WB analysis of pig brain using 67163-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67163-1-Ig (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 67163-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67163-1-Ig (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 67163-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67163-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 67163-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human colon cancer using 67163-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using HIP1 antibody (67163-1-Ig, Clone: 3D3G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, pig brain tissue, mouse brain tissue, A549 cells, PC-3 cells
• Positive IHC detected in: human colon cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human colon cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67163-1-Ig targets HIP1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, pig samples.

• Tested Reactivity: Human, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HIP1 fusion protein Ag17982
• Full Name: huntingtin interacting protein 1
• Calculated Molecular Weight: 1037 aa, 116 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC110545
• Gene Symbol: HIP1
• Gene ID (NCBI): 3092
• RRID: AB_2882459
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIP1 (huntingtin interacting protein-1) is a endocytic protein that plays a role in clathrin-mediated endocytosis and trafficking. HIP1 can interact with huntingtin (HTT) protein whose mutations cause Huntington's disease (HD), thus gets involved in neurodegeneration. By stabilizing receptor tyrosine kinases following ligand-induced endocytosis, HIP1 may act to promote cell survival. HIP1 is overexpressed in a variety of human cancers and may be a potential predictor for cancer progression and prognosis.