Anti-Human HLA class II Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98748-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human HLA class II Rabbit RecAb (98748-1-RR, Clone:253538A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), and 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were then stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98748-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human HLA class II Rabbit RecAb (98748-1-RR, Clone:253538A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), and 0.25 ug Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (65560-1-MR, Clone: L243). Cells were then stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

HLA class II Uni-rAb^® Recombinant Antibody for FC

Cat No. 98748-1-RR

Clone No.253538A1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

MHC II, HLA-D, MHC class II

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98748-1-RR targets HLA class II in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  unknow

• Full Name: HLA class II
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system (PMID: 667938; 3375250). This antibody can recognize HLA-DR, -DP, and -DQ.