HSPA4 Polyclonal antibody

HSPA4 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21206-1-AP

Synonyms

APG 2, APG2, Heat shock 70 kDa protein 4, heat shock 70kDa protein 4, HS24/P52, HSP70.4, hsp70RY, HSPA4, RY



Tested Applications

Positive WB detected inC6 cells, NIH/3T3 cells, mouse testis tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21206-1-AP is HSPA4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSPA4 fusion protein Ag15581
Full Name heat shock 70kDa protein 4
Calculated molecular weight 840 aa, 94 kDa
Observed molecular weight 110-120 kDa
GenBank accession numberBC110861
Gene symbol HSPA4
Gene ID (NCBI) 3308
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPA4 (also known as Apg-2, HSP70RY) is a 110 kDa cytosolic protein, a member of the heat-shock protein 110 (HSP110) subfamily of HSP70 proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA4 is ubiquitously expressed and its expression is not heat inducible. Overexpression of HSPA4 has been reported in some leukemia and solid tumors. This antibody well recognized the endogenous HSPA4 protein in mouse brain/ testis tissues. (PMID: 21487003)

Protocols

Product Specific Protocols
WB protocol for HSPA4 antibody 21206-1-APDownload protocol
IHC protocol for HSPA4 antibody 21206-1-APDownload protocol
IF protocol for HSPA4 antibody 21206-1-APDownload protocol
IP protocol for HSPA4 antibody 21206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Med Sci

Exercise combined with trimetazidine improves anti-fatal stress capacity through enhancing autophagy and heat shock protein 70 of myocardium in mice.

Authors - Lingjun Jiang
humanELISA

Eur J Clin Invest

Salivary monitoring related to major surgery.

Authors - Ullmann Yehuda Y
mouseWB

Int J Biochem Cell Biol

Folate deprivation induces cell cycle arrest at G0/G1 phase and apoptosis in hippocampal neuron cells through down-regulation of IGF-1 signaling pathway.

Authors - Yang Yang