ChromoTek Halo-Trap Agarose beads

Halo-Trap Agarose are affinity beads for IP of Halo-tag proteins. It comprises a Halo-tag Nanobody /VHH conjugated to agarose beads.

Specificity

Halo-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Cat no : ota

Synonyms

Halo-Trap, Halo tag, dehalogenase



Product Information

Halo-Trap Agarose are affinity beads for IP of Halo-tag proteins. It comprises a Halo-tag Nanobody /VHH conjugated to agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of 2 nM
Specificity/TargetHalo-tag
Coupled VHHRecombinant, monoclonal anti-Halo single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.1 mM citric acid pH 3.0
Storage Buffer20% EtOH
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Publications

ApplicationTitle
IP

ACS Chem Biol

Modulation of Phosphoprotein Activity by Phosphorylation Targeting Chimeras (PhosTACs).

Authors - Po-Han Chen
IP

Nat Commun

Galectin-9 restricts hepatitis B virus replication via p62/SQSTM1-mediated selective autophagy of viral core proteins.

Authors - Kei Miyakawa

Nat Chem Biol

Bright and stable luminescent probes for target engagement profiling in live cells.

Authors - N Connor Payne
WB,IP

Mol Cell

Poly(ADP-ribose) drives condensation of FUS via a transient interaction.

Authors - Kevin Rhine

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Anastasija (Verified Customer) (02-14-2022)

I used the Halo-trap agarose to perform nuclear co-IP of Halo-tagged proteins, and after a bit of optimization of the IP protocol I achieved very good pulldown efficiency with high amounts of bait and interactors, and low background. An added benefit is that upon elution there is no IgG co-eluting with the target proteins, which is normally a problem in conventional IP approaches using IgG. I am really happy with this product, and will continue using it in future experiments.

  • Applications: Immunoprecipitation
  • Cell Tissue Type: HEK293T