Cat no : ota
Halo-Trap Agarose are affinity beads for IP of Halo-tag proteins. It comprises a Halo-tag Nanobody /VHH conjugated to agarose beads.
|Applications||IP, CoIP, ChIP, RIP|
|Affinity||Dissociation constant KD of 2 nM|
|Coupled VHH||Recombinant, monoclonal anti-Halo single domain antibody (sdAb) fragment|
|Beads||~ 90 µm (cross-linked 4 % agarose beads)|
|Elution buffer||SDS sample buffer|
0.1 mM citric acid pH 3.0
|Storage Buffer||20% EtOH|
|Storage Condition||Upon receipt store at +4°C. Do not freeze!|
|SDS Halo-Trap Agarose (PDF)|
|Protocol Halo-Trap Agarose (PDF)|
|Troubleshooting guide immunoprecipitation (IP) (PDF)|
ACS Chem Biol
Modulation of Phosphoprotein Activity by Phosphorylation Targeting Chimeras (PhosTACs).
Galectin-9 restricts hepatitis B virus replication via p62/SQSTM1-mediated selective autophagy of viral core proteins.
Nat Chem Biol
Bright and stable luminescent probes for target engagement profiling in live cells.
Poly(ADP-ribose) drives condensation of FUS via a transient interaction.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Anastasija (Verified Customer) (02-14-2022)
I used the Halo-trap agarose to perform nuclear co-IP of Halo-tagged proteins, and after a bit of optimization of the IP protocol I achieved very good pulldown efficiency with high amounts of bait and interactors, and low background. An added benefit is that upon elution there is no IgG co-eluting with the target proteins, which is normally a problem in conventional IP approaches using IgG. I am really happy with this product, and will continue using it in future experiments.