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ChromoTek Halo-Trap Magnetic Particles M-270

The ChromoTek Halo-Trap Magnetic Particles M-270 consists of an anti-Halo-tag Nanobody (VHH), which is covalently bound to Magnetic Particles M-270. Halo-Trap Magnetic Particles M-270 is used to immunoprecipitate Halo-tag proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc. in the presence or absence of a covalently bound ligand. The interaction between Halo-Trap and the Halo-tag protein is reversible.

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Particles M-270

Specificity

Halo-tag

Cat no : otd

Synonyms

Halo Trap Magnetic Particles M-270, Halo-Trap Magnetic Particles M-270, Halo-Trap, Halo, Halo tag, otd

Validation Data Gallery

  • Halo-Trap Magnetic Particles M-270 was used for immunoprecipitation of Halo-tag protein from HEK293T cell lysate and elution with 2x SDS-sample buffer. Coomassie blue staining shows elution of enriched Halo-tag protein. Western blot was probed with Halo antibody [28A8] (28a8) and Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104] (sms1AF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

    Halo-Trap Magnetic Particles M-270 was used for immunoprecipitation of Halo-tag protein from HEK293T cell lysate and elution with 2x SDS-sample buffer. Coomassie blue staining shows elution of enriched Halo-tag protein. Western blot was probed with Halo antibody [28A8] (28a8) and Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104] (sms1AF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

  • Halo-Trap Magnetic Particles M-270 was used for immunoprecipitation of Halo-tag protein from HEK293T cell lysate and elution with 2x SDS-sample buffer. Coomassie blue staining shows elution of enriched Halo-tag protein. L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

    Halo-Trap Magnetic Particles M-270 was used for immunoprecipitation of Halo-tag protein from HEK293T cell lysate and elution with 2x SDS-sample buffer. Coomassie blue staining shows elution of enriched Halo-tag protein. L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

Product Information

The ChromoTek Halo-Trap Magnetic Particles M-270 consists of an anti-Halo-tag Nanobody (VHH), which is covalently bound to Magnetic Particles M-270. Halo-Trap Magnetic Particles M-270 is used to immunoprecipitate Halo-tag proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc. in the presence or absence of a covalently bound ligand. The interaction between Halo-Trap and the Halo-tag protein is reversible.

ClonalityMonoclonal
ApplicationsIP, CoIP, ChIP, RIP
HostAlpaca
ConjugateMagnetic Particles M-270
RRIDAB_2894835
Coupled VHHAnti-Halo-tag single domain antibody fragment (VHH, Nanobody)
ReactivityHalo-tag
Bead size2.8 µm
Binding capacity1.25 µg of recombinant Halo-tag per 25 µL bead slurry
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5 or 0.1 M citric acid pH 3.0
Storage buffer1x PBS, Preservative: 0.09 % sodium azide
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Rick (Verified Customer) (07-22-2022)

It worked well, just few troubleshooting. used succesfully on cancer line and HEK cells

  • Applications: Protein purification
  • Cell Tissue Type: HEK & cancer line