ChromoTek Halo-Trap Magnetic Particles M-270 Kit

The ChromoTek Halo-Trap Magnetic Particles M-270 Kit consists of an anti-Halo-tag Nanobody (VHH), which is covalently bound to Magnetic Particles M-270. Halo-Trap Magnetic Particles M-270 Kit is used to immunoprecipitate Halo-tag proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc. in the presence or absence of a covalently bound ligand. The interaction between Halo-Trap and the Halo-tag protein is reversible.

Specificity

Halo-Tag

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Magnetic Particles M-270

Cat no : otdk

Synonyms

Halo Trap Magnetic Particles M-270, Halo-Trap Magnetic Particles M-270, Halo-Trap Magnetic Particles M-270 kit, otdk, otdk-20, Halo, Halo tag, Halo-tag, otdk 20

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Validation Data Gallery

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  Halo-Trap Magnetic Particles M-270 was used for immunoprecipitation of Halo-tag protein from HEK293T cell lysate and elution with 2x SDS-sample buffer. Coomassie blue staining shows elution of enriched Halo-tag protein. Western blot was probed with Halo antibody [28A8] (28a8) and Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104] (sms1AF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

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  Halo-Trap Magnetic Particles M-270 was used for immunoprecipitation of Halo-tag protein from HEK293T cell lysate and elution with 2x SDS-sample buffer. Coomassie blue staining shows elution of enriched Halo-tag protein. L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

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Product Information

The ChromoTek Halo-Trap Magnetic Particles M-270 Kit consists of an anti-Halo-tag Nanobody (VHH), which is covalently bound to Magnetic Particles M-270. Halo-Trap Magnetic Particles M-270 Kit is used to immunoprecipitate Halo-tag proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc. in the presence or absence of a covalently bound ligand. The interaction between Halo-Trap and the Halo-tag protein is reversible.

• Description: The Halo-Trap Magnetic Particles M-270 Kit contains Halo-Trap Magnetic Particles M-270, lysis, wash, and elution buffers for efficient immunoprecipitation of Halo-tagged proteins and their interacting factors.

  • Halo-Trap immunoprecipitates Halo-fusion proteins even when already covalently bound to Halo-ligands, i.e. after labelling etc.

  • Ready-to-use

  • No heavy & light antibody chains on the SDS-PAGE

  • Stable under harsh washing conditions

  • Suitable for downstream mass spec analysis

• Applications: IP, CoIP, ChIP, RIP
• Specificity/Target: Halo-tag (modified variant of the bacterial haloalkane dehalogenase enzyme from Rhodococcus rhodochrous) in the absence or presence of covalently bound chloralkane-based ligands.
• Binding capacity: 1.25 μg of recombinant Halo-tag per 25 μL bead slurry
• Conjugate: Magnetic Particles M-270, size: 2.8 µm; high throughput-compatible
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Wash buffer compatibility: 2 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 2 % Triton X-100
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 2 nM
• Compatibility with mass spectrometry: The Halo-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2894835
• Storage Buffer: 20% ethanol
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Kit components

Component	Description
Dilution buffer	Dilution of cell lysate
Elution buffer	For acidic elution
Lysis buffer	Optimized for cytoplasmatic proteins and mammalian cell lysis
RIPA buffer	Optimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer	Removal of unwanted proteins, peptides, etc.
Halo-Trap Magnetic Particles M-270	20 reactions (500 µL slurry)

Other Formats

Halo-Trap Agarose kit

Halo-Trap Agarose

Halo-Trap Magnetic Agarose Kit

Halo-Trap Magnetic Agarose

Halo-Trap Magnetic Particles M-270