ChromoTek Halo-Trap Agarose beads, kit

Halo-Trap Agarose are affinity beads for IP of Halo-tag fusion proteins. It comprises a Halo-tag Nanobody /VHH conjugated to agarose beads.

Specificity

Halo-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Cat no : otak

Synonyms

Halo-Trap, Halo tag, dehalogenase



Product Information

Halo-Trap Agarose are affinity beads for IP of Halo-tag fusion proteins. It comprises a Halo-tag Nanobody /VHH conjugated to agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
Clonality Monoclonal
HostAlpaca
AffinityDissociation constant KD of 2 nM
Specificity/TargetHalo-tag
Coupled VHHRecombinant, monoclonal anti-Halo single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.1 mM citric acid pH 3.0
Storage Buffer20% EtOH
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Kit components

ComponentDescription
Halo-Trap Agarose beads 20 reactions (500 µL)
Lysis buffer Optimized for cytoplasmatic proteins and mammalian cell lysis
RIPA buffer Optimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution buffer Dilution of cell lysate
Elution buffer For acidic elution

Publications

ApplicationTitle
CoIP

Dev Cell

The Rag GTPase Regulates the Dynamic Behavior of TSC Downstream of Both Amino Acid and Growth Factor Restriction.

Authors - Shu Yang