ChromoTek Halo-Trap Agarose beads, kit

Halo-Trap Agarose are affinity beads for IP of Halo-tag proteins. It comprises a Halo-tag Nanobody /VHH conjugated to agarose beads.

Specificity

Halo-Tag

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Agarose

Publications

1

Cat no : otak

Synonyms

Halo-Trap, Halo tag, dehalogenase

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Validation Data Gallery

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  Immunoprecipitation of Halo-tag protein with Halo-Trap.I: Input, FT: Flow-through, B: Bound.

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Product Information

Halo-Trap Agarose are affinity beads for IP of Halo-tag proteins. It comprises a Halo-tag Nanobody /VHH conjugated to agarose beads.

• Description: The Halo-Trap Agarose Kit contains Halo-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of Halo-tagged proteins and their interacting factors.

  • Halo-Trap immunoprecipitates Halo-fusion proteins even when already covalently bound to Halo-ligands, i.e. after labelling etc.

  • Fast, reliable & efficient one-step immunoprecipitation

  • No heavy & light antibody chains

  • Bound Halo-fusion protein can be eluted without protease

  • Suitable for downstream mass spec analysis

• Applications: IP, CoIP, ChIP, RIP
• Specificity/Target: Halo-tag (modified variant of the bacterial haloalkane dehalogenase enzyme from Rhodococcus rhodochrous) in the absence or presence of covalently bound chloralkane-based ligands.
• Binding capacity: 7.5-10 μg of recombinant Halo-tag per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Wash buffer compatibility: 4 M urea, 1 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 2 nM
• Compatibility with mass spectrometry: The Halo-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2827595
• Storage Buffer: 20% ethanol
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Kit components

Component	Description
Halo-Trap Agarose beads	20 reactions (500 µL)
Lysis buffer	Optimized for cytoplasmatic proteins and mammalian cell lysis
RIPA buffer	Optimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer	Removal of unwanted proteins, peptides, etc.
Dilution buffer	Dilution of cell lysate
Elution buffer	For acidic elution

Other Formats

Halo-Trap Agarose

Halo-Trap Magnetic Agarose Kit

Halo-Trap Magnetic Agarose

Halo-Trap Magnetic Particles M-270 Kit

Halo-Trap Magnetic Particles M-270

Publications

Application	Title
CoIP	Dev Cell; The Rag GTPase Regulates the Dynamic Behavior of TSC Downstream of Both Amino Acid and Growth Factor Restriction.; Authors - Shu Yang