Histone H2B Polyclonal antibody, PBS Only

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  WB analysis of HEK-293T using 27740-1-AP (same clone as 27740-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27740-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 27740-1-AP (same clone as 27740-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27740-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 27740-1-AP (same clone as 27740-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27740-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 27740-1-AP (same clone as 27740-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using Histone H2B antibody (27740-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27740-1-PBS in a different storage buffer formulation.

• 

  ChIP experiment of HEK-293 using 27740-1-AP (same clone as 27740-1-PBS)

  Chromatin was prepared from HEK-293 cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 18 µg of cross-linked chromatin, 5 µg of Histone H2B Antibody (27740-1-AP) or 5 ug of Normal Rabbit IgG (30000-0-AP), and 30 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region. This data was developed using the same antibody clone with 27740-1-PBS in a different storage buffer formulation.

Histone H2B Polyclonal Antibody for WB, IF/ICC, ChIP, Indirect ELISA

Cat No. 27740-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ChIP, Indirect ELISA

Synonyms

H2B3B, HIST3H2BB, H2B-clustered histone 26, H2BC26, H2B.U histone 1

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Product Information

27740-1-PBS targets Histone H2B in WB, IF/ICC, ChIP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Histone H2B fusion protein Ag26901
• Full Name: histone cluster 3, H2bb
• Observed Molecular Weight: 15-20 kDa
• GenBank Accession Number: BC100855
• Gene Symbol: Histone H2B
• Gene ID (NCBI): 128312
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N257
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST3H2BB is a replication-dependent histone that is a member of the histone H2B family.