Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, A549 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, Neuro-2a cells, RAW 264.7 cells, NCI-H1299 cells |
| Positive IHC detected in | human ovary tumor tissue, human breast cancer tissue, rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66549-1-Ig targets HuR/ELAVL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2249 Product name: Recombinant human HuR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-326 aa of BC003376 Sequence: MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEVIKDANLYISGLPRTMTQKDVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEEAITSFNGHKPPGSSEPITVKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRFRFSPMGVDHMSGLSGVNVPGNASSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAMAIASLNGYRLGDKILQVSFKTNKSHK Predict reactive species |
| Full Name | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R) |
| Calculated Molecular Weight | 326 aa, 36 kDa |
| Observed Molecular Weight | 34-37 kDa |
| GenBank Accession Number | BC003376 |
| Gene Symbol | HuR/ELAVL1 |
| Gene ID (NCBI) | 1994 |
| RRID | AB_2881911 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15717 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HuR/ELAVL1 antibody 66549-1-Ig | Download protocol |
| IHC protocol for HuR/ELAVL1 antibody 66549-1-Ig | Download protocol |
| WB protocol for HuR/ELAVL1 antibody 66549-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HuR/ELAVL1 Monoclonal antibody (catalog no. 66549-1-Ig) has 8 total citations spanning journals including Cell Death & Disease, Cell Signaling, Biochimica et Biophysica Acta, Biology, Molecular and Cellular Endocrinology, Oncology Letters, Journal of International Medical Research, and eLife. Research fields cover colorectal cancer tumorigenesis, inflammatory gene regulation, melanoma progression, ferroptosis in peripheral artery disease, diabetic osteoporosis, renal cancer drug resistance, gastric cancer migration, and intercellular communication via intercellsomes.
| Species | Application | Title |
|---|---|---|
Cell Death Dis LncGMDS-AS1 promotes the tumorigenesis of colorectal cancer through HuR-STAT3/Wnt axis
| ||
Cell Signal Butyrate mediated regulation of RNA binding proteins in the post-transcriptional regulation of inflammatory gene expression. | ||
Biology (Basel) Deciphering the Role of Ferroptosis in the Pathogenesis of Peripheral Artery Disease Myopathy | ||
Biochim Biophys Acta Mol Cell Res IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5 | ||
Mol Cell Endocrinol ELAV-like RNA binding protein 1 regulates osteogenesis in diabetic osteoporosis: Involvement of divalent metal transporter 1. | ||
Oncol Lett Stress granules affect the sensitivity of renal cancer cells to sorafenib by sequestering and stabilizing COX‑2 mRNA |
