CoraLite®594-conjugated HuR/ELAVL1 Monoclonal antibody

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  IF Staining of L02 using CL594-66549

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using CoraLite®594-conjugated HuR/ELAVL1 antibody (CL594-66549, Clone: 1A11H2 ) at dilution of 1:100, CL488-Phalloidin (green).

• Featured Product
• KD/KO Validated

HuR/ELAVL1 Monoclonal Antibody for IF/ICC

Cat No. CL594-66549

Clone No.1A11H2

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF/ICC

Synonyms

ELAV like protein 1, ELAV1, ELAVL1, Hu antigen R, Hua, HuR, MelG

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Tested Applications

• Positive IF/ICC detected in: L02 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66549 targets HuR/ELAVL1 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2249

  Product name: Recombinant human HuR protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-326 aa of BC003376

  Sequence: MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEVIKDANLYISGLPRTMTQKDVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEEAITSFNGHKPPGSSEPITVKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRFRFSPMGVDHMSGLSGVNVPGNASSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAMAIASLNGYRLGDKILQVSFKTNKSHK

• Full Name: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
• Calculated Molecular Weight: 326 aa, 36 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC003376
• Gene Symbol: HuR/ELAVL1
• Gene ID (NCBI): 1994
• RRID: AB_2883602
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15717
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.