Filter: AllWBIHCIF

WB analysis using 66549-1-Ig (same clone as 66549-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66549-1-Ig (HuR/ELAVL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 66549-1-Ig (HuR/ELAVL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 66549-1-Ig (same clone as 66549-1-PBS)
WB result of HuR/ELAVL1 antibody (66549-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HuR transfected HEK-293 cells. This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.
WB analysis using 66549-1-Ig (same clone as 66549-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66549-1-Ig (HuR/ELAVL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66549-1-Ig (same clone as 66549-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66549-1-Ig (HuR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 66549-1-Ig (same clone as 66549-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66549-1-Ig (HuR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66549-1-Ig (same clone as 66549-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66549-1-Ig (HuR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 66549-1-Ig (same clone as 66549-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66549-1-Ig (HuR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66549-1-Ig (same clone as 66549-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HuR/ELAVL1 antibody (66549-1-Ig, Clone: 1A11H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66549-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

HuR/ELAVL1 Monoclonal antibody, PBS Only

HuR/ELAVL1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66549-1-PBS

Clone No.1A11H2

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ELAV like protein 1, ELAV1, ELAVL1, Hu antigen R, Hua, HuR, MelG

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66549-1-PBS targets HuR/ELAVL1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity	Human, mouse, rat
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag2249 Product name: Recombinant human HuR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-326 aa of BC003376 Sequence: MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEVIKDANLYISGLPRTMTQKDVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEEAITSFNGHKPPGSSEPITVKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRFRFSPMGVDHMSGLSGVNVPGNASSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAMAIASLNGYRLGDKILQVSFKTNKSHK Predict reactive species
Full Name	ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
Calculated Molecular Weight	326 aa, 36 kDa
Observed Molecular Weight	34-37 kDa
GenBank Accession Number	BC003376
Gene Symbol	HuR/ELAVL1
Gene ID (NCBI)	1994
RRID	AB_2881911
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q15717
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.