Recombinant Human CCL8/MCP-2 protein (rFc Tag)(HPLC verified)
Species
Human
Purity
>90 %, SDS-PAGE
>90%, SEC-HPLC
Tag
rFc Tag
Activity
not tested
Cat no : Eg5089
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE >90%, SEC-HPLC |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human CCL8 protein Gln24-Pro99 (Accession# P80075) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 6355 |
| Accession | P80075 |
| PredictedSize | 35.2 kDa |
| SDS-PAGE | 35-40 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in 20 mM PB, 150 mM NaCl, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
CCL8 (also known as MCP2) is implicated in various inflammatory processes. In bone marrow transplantation, circulating CCL8 levels positively correlate with severity of graft-versus-host disease.
References:
1. Vilgelm AE, et al. (2019). Front Immunol. 10: 333. 2. Yamamoto M, et al. (2011). Exp Hematol. 39: 1101–1112. 3. Klementowicz JE, et al. (2017). J Immunol. 199: 27–32. 4. Hori T, et al. (2008). Blood, 111: 4403–4412.