Recombinant Human CTLA4 protein (His Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
His Tag
Activity
not tested
Cat no : Eg0972
Validation Data Gallery
Product Information
Purity | >90 %, SDS-PAGE |
Endotoxin | <1.0 EU/μg protein, LAL method |
Activity |
Not tested |
Expression | HEK293-derived Human CTLA4 protein Lys36-Asp161 (Accession# P16410) with a His tag at the C-terminus. |
GeneID | 1493 |
Accession | P16410 |
PredictedSize | 14.6 kDa |
SDS-PAGE | |
Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses. Besides the full-length transmembrane form, CTLA-4 also exists in a truncated soluble form (sCTLA-4).
References:
1. Brunet, J F et al. Nature vol. 328,6127 (1987): 267-70. 2. Harper, K et al. Journal of immunology (Baltimore, Md. : 1950) vol. 147,3 (1991): 1037-44.