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Recombinant Human Fc Epsilon RI Alpha protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2022

Validation Data Gallery

  • Purity of Recombinant Human Fc Epsilon RI Alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human Fc Epsilon RI Alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human Fc Epsilon RI Alpha protein Val26-Gln205 (Accession# P12319) with a rabbit IgG Fc tag at the C-terminus.
GeneID 2205
Accession P12319
PredictedSize 47.3 kDa
SDS-PAGE 52-80 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Fc fragment of IgE, high affinity I, receptor for, alpha polypeptide, also known as high affinity immunoglobulin epsilon receptor subunit alpha, FCER1A and FCE1A, is a single-pass type I membrane protein which contains 2 immunoglobulin-like domains. FCER1A is a subunit of the IgE receptor, which is composed of one glycosylated alpha (FCER1A), one beta (FCER1B), and two gamma (FCER1G) subunits. The high affinity IgE receptor plays a central role in allergic disease, coupling allergen and mast cells to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma.

References:

1. S S Saini, et al. (2001) J Allergy Clin Immunol. May;107(5):832-41.
2. Natalija Novak, et al. (2003) J Clin Invest. Apr;111(7):1047-56. 
3. M C Seminario, et al. (1999) J Immunol. Jun 1;162(11):6893-900.