Recombinant Human IL-17RD protein (rFc Tag)(HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg4973

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Validation Data Gallery

• 

  Purity of Recombinant Human IL-17RD was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Human IL-17RD was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human IL-17RD protein Cys17-Arg299 (Accession# Q8NFM7) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 54756
• Accession: Q8NFM7
• PredictedSize: 58.1 kDa
• SDS-PAGE: 70-80 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Pro-inflammatory cytokines of the interleukin-17 (IL-17) family are secreted by cells of the activated immune system. The IL-17 cytokine family consists of six ligands (IL-17A-F), which signal through five receptors (IL-17RA-E). IL-17RD negatively regulates IL-17A-induced activation of NF-κB and the expression of pro-inflammatory genes such as IL-6. IL-17RD coordinates these regulatory effects by negatively regulating Act1/TRAF6 binding and disrupting the IL-17RA/Act1/TRAF6 signalling axis.

References:

1. Mellett M, et al. (2013). Nat Commun. 3:1119.
2. Miraoui H, et al. (2013). Am J Hum Genet. 92(5):725-43.
3. Liu X, et al. (2020). Int Arch Allergy Immunol. 181(8):618-623.