Recombinant Human JAM-A/Junctional Adhesion Molecule A protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg1372

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Validation Data Gallery

• 

  Purity of Recombinant Human JAM-A was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human JAM-A protein Ser28-Val238 (Accession# Q9Y624-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 50848
• Accession: Q9Y624-1
• PredictedSize: 49.2 kDa
• SDS-PAGE: 45-60 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Junctional Adhesion Molecule A (JAM-A) is a member of the JAM family of cell adhesion molecules. In epithelial cells, JAM-A signaling modulates cell migration in a β1 integrin-dependent manner. JAM-A is a crucial regulator of leukocyte extravasation and is upregulated in human viral fibrosis. JAM-A functions as a female microglial tumor suppressor in glioblastoma.

References:

1. Ebnet K. (2017). Physiol Rev. 97(4):1529-1554.
2. Severson EA. et al. (2009). Curr Opin Cell Biol. 21(5):701-70.
3. Schmitz SM. et al. (2022). Liver Int. 42(5):1185-1203.
4. Turaga SM. et al. (2020). Neuro Oncol. 22(11):1591-1601.