Recombinant Human LYVE-1 protein (rFc Tag)

Species

Human

Purity

>85 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg1615

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Validation Data Gallery

• 

  Purity of Recombinant Human LYVE-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >85 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human LYVE-1 protein Leu20-Thr238 (Accession# Q9Y5Y7) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 10894
• Accession: Q9Y5Y7
• PredictedSize: 49.9 kDa
• SDS-PAGE: 70-90 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

The lymphatic vessel endothelial hyaluronan receptor-1 (LYVE1) gene is located on chromosome 11p15.4 and comprises six exons. LYVE1 is notably expressed on lymphatic endothelial cells (LEC) and selected macrophages, but not on microglia. In the periphery, macrophage populations lining the blood vessels or nerve bundles have been characterized as LYVE1hi and LYVE1lo, respectively.

References:

1. Siret C, et al. (2022). Nat Commun. 13(1):7366.
2. Feng Z, et al. (2023). Dis Markers. 6978234.