Recombinant Human CD73 protein (rFc Tag)

Species

Human

Purity

>85 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2974

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Validation Data Gallery

• 

  Purity of Recombinant Human CD73 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >85 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human CD73 protein Trp27-Lys547 (Accession# P21589-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 4907
• Accession: P21589-1
• PredictedSize: 83.8 kDa
• SDS-PAGE: 80-95 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues . CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates. In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells. CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression .

References:

1.Colgan, Sean P et al. Purinergic signalling vol. 2,2 (2006): 351-60.
2.Jin, Dachuan et al. Cancer research vol. 70,6 (2010): 2245-55.
3.Resta, R et al. Immunological reviews vol. 161, (1998): 95-109.
4.Thomson, L F et al. Tissue antigens vol. 35,1 (1990): 9-19. 
5.Jin, Dachuan et al. Cancer research vol. 70,6 (2010): 2245-55.