Recombinant Human Podoplanin protein (rFc Tag)(HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg5024

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Validation Data Gallery

• 

  Purity of Recombinant Human Podoplanin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Human Podoplanin was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human Podoplanin protein Ala23-Leu131 (Accession# Q86YL7-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 10630
• Accession: Q86YL7-1
• PredictedSize: 37.4 kDa
• SDS-PAGE: 48-68 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Podoplanin (PDPN) is a transmembrane glycoprotein that plays a role in a variety of biological processes including regulation of blood-lymphatic vessel development, cerebral vascular pattern and integrity, cell motility, tumorigenesis and metastasis, and mediates tumor cell-induced platelet aggregation in different cancer types. It is a lymphatic marker because the expression of podoplanin has been detected in lymphatic but not blood vascular endothelium, and is useful as the marker of tumor-associated Lymphangiogenesis. When expressed in keratinocytes, PDPN induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion.

References:

1. Ukaji T, et al . Cancer Sci. 2021; 112(6):2299-2313.                                                                        
2. Krishnan H, et al. J Biol Chem. 2013 ;288(17):12215-21.                                                              
3. Wang X, et al. Cancer Gene Ther. 2023 ;30(2):345-357.                                                        
4.Astarita JL, et al. Front Immunol. 2012; 3:283.