Recombinant Human Thrombomodulin protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0944

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Validation Data Gallery

• 

  Purity of Recombinant Human Thrombomodulin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human Thrombomodulin protein Ala19-Ser515 (Accession# P07204) with a His tag at the C-terminus.
• GeneID: 7056
• Accession: P07204
• PredictedSize: 53.2 kDa
• SDS-PAGE: 70-100 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood. Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation. Mutations in the gene of thrombomodulin are a cause of thromboembolic disease, also known as inherited thrombophilia.

References:

1. N L Esmon, et al. (1987) Semin Thromb Hemost. 13(4):454-63.
2. A K Ohlin, et al. (1997) Thromb Haemost. 78(1):396-400.
3. M C Boffa, et al. (1998) Lupus.7 Suppl 2:S120-5.
4. Georgia Anastasiou, et al. (2012) Blood Coagul Fibrinolysis. 23(1):1-10.