IFNAR2 Polyclonal antibody

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  WB analysis using 10522-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10522-1-AP (IFNAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of MCF-7 using 10522-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug IFNAR2 Polyclonal antibody (10522-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

IFNAR2 Polyclonal Antibody for WB, FC (Intra), ELISA

Cat No. 10522-1-AP

Publications(5)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, FC (Intra), IP, ELISA

Synonyms

IFN-alpha binding protein, IFNABR, IFN R 2, IFN R, IFN alpha/beta receptor 2

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, MCF-7 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

10522-1-AP targets IFNAR2 in WB, IF, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFNAR2 fusion protein Ag0191
• Full Name: interferon (alpha, beta and omega) receptor 2
• Calculated Molecular Weight: 331 aa, 37 kDa
• Observed Molecular Weight: 58-70 kDa
• GenBank Accession Number: BC002793
• Gene Symbol: IFNAR2
• Gene ID (NCBI): 3455
• RRID: AB_10694421
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48551
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	EMBO Rep; Degradation of WTAP blocks antiviral responses by reducing the m 6 A levels of IRF3 and IFNAR1 mRNA; Authors - Yong Ge
human	WB,IP	J Virol; African swine fever virus pH240R enhances viral replication via inhibition of the type I IFN signaling pathway; Authors - Guangqiang Ye
human	WB	J Virol; Herpes simplex virus 1 UL36USP antagonizes type I IFN-mediated antiviral innate immunity.; Authors - Hui Yuan
human	WB,IP,IF	J Biol Chem; Protein kinase A suppresses anti-proliferative effect of interferon-α in hepatocellular carcinoma by activation of protein tyrosine phosphatase SHP2; Authors - Yuwen Sheng
pig	WB	PLoS Pathog; African swine fever virus pB318L, a trans-geranylgeranyl-diphosphate synthase, negatively regulates cGAS-STING and IFNAR-JAK-STAT signaling pathways; Authors - Xiaohong Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Angie (Verified Customer) (04-09-2024)

The antibody detected a band around 70 kD at dilution of 1:2000 incubated at room temperature for 2 hour followed by secondary antibody donkey-anti-rabbit (Alexa Fluor 800) at 1:20000 dilution, incubated for 1 hour at room temperature.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: A549, HEK293

IFNAR2 Antibody Western Blot validation (1:2000 dilution) in A549, HEK293 (Cat no:10522-1-AP)